Biolegend, 697302, Purified anti-ATF7 Antibody, 100μg

Activating Transcription Factor 7 (ATF7) is a member of the ATF/cAMP response element-binding protein family. Among these ATF family members, ATF7 shares highly structurally conserved N-terminal activation domain and the C-terminal DNA binding/dimerization domain with ATF2. ATF7 has no intrinsic transcriptional activity and forms functional heterodimers with c-Jun or c-Fos proteins. Upon stimulation, such as pathogen infection and various stresses, ATF7 is phosphorylated by stress‐activated protein kinase p38 at Thr51 and Thr53 and in turn resulted in the activation of ATF7 target genes. In the absence of stress, ATF silences targets genes by recruiting histone methyltransferases, leading to the formation of heterochromatin. ATF7 deficient mice die soon after birth. Double knockout mutations of ATF2 and ATF7 lead to prenatal lethality with abnormalities in liver and heart.
100μg
Verified Reactivity: Human, Mouse
Antibody Type: Monoclonal
Host Species: Rat
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality testedICC - Verified
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.2 - 2.0 µg per ml. For immunocytochemistry, a concentration range of 0.5 - 2.0 µg per ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
RRID: AB_2687154 (BioLegend Cat. No. 697302)
Structure: 494 amino acids with a predicted molecular weight of 53 kD. Contains a N-terminal transactivation domain and a C-terminal bZIP DNA binding domain.
Distribution: Nucleus
Function: ATF7 is a transcription factor belonging to the the ATF/cAMP response element-binding protein family. ATF7 is involved in early cell signaling and is activated in response to pathogen infection and stress.
Interaction: Forms a homodimer or a heterodimer with c-Jun, c-Fos, and other ATF family proteins. Interacts with JNK2, TAF12, TAF4, and MAPK9.
Biology Area: Cell Biology
Antigen References: 1. Yoshida K, Ishii S. 2016. Cell Cycle. 15:3. 2. Yoshida K, et al. 2015. Nat. Immunol. 16:1034. 3. Schaeffer E, et al. 2015. Cell Cycle. 14:2655. 4. Shivers RP, et al. 2010. PLoS Genet. 6:e1000892. 5. Camuzeaux B, et al. 2008. J. Mol. Biol. 384:980. 6. Breitwieser W, et al. 2007. Genes. Dev. 21:2069.
Gene ID: 11016
UniProt: View information about ATF7 on UniProt.org
Clone: W16152A
Regulatory Status: RUO
Other Names: Cyclic AMP-dependent transcription factor ATF-7, Transcription factor ATF-A
Isotype: Rat IgG2a, κ