Biolegend, 697702, Purified anti-TSC2 Antibody, 100μg

Tuberous sclerosis 2 (TSC2) forms the tuberous sclerosis complex with TSC1 and functions as a crucial negative regulator of the Rheb/mTOR pathway. mTOR belongs to the PI3K-related kinase family and regulates protein translation, cell proliferation, and cell differentiation. Activation of mTOR requires binding to the active small GTPase Ras homolog enriched in brain (Rheb). TSC2 acts as a GTPase activating protein (GAP) and inhibits mTORC1 through converting active GTP-bound Rheb to inactive GDP-bound Rheb. Phosphorylation of TSC2 by AKT leads to inactivation of the tuberous sclerosis complex and in turn results in the activation of the Rheb/mTOR pathway. Mutations in the TSC2 gene lead to hyperactivation of the Rheb/mTOR pathway and are associated with the autosomal dominant disorder tuberous sclerosis and pulmonary lymphangioleiomyomatosis (LAM).
100μg
Verified Reactivity: Human, Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Human TSC2 recombinant protein (1508-1807 amino acid) expressed in E. coli.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested KO/KD-WB - Verified
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.2 - 2.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: This clone does not work for IF application (in house tested). Antibody specificity was confirmed by knock-down of TSC2 using siRNA (in-house tested).
RRID: AB_2687204 (BioLegend Cat. No. 697701) AB_2687188 (BioLegend Cat. No. 697702)
Structure: 1807 amino acids with a predicted molecular weight of 200 kD. Contains a Rap-GAP domain responsible for converting GTP-bound Rheb to its GDP-bound form.
Distribution: Cytoplasm. Membrane.
Function: TSC2 is a GTPase activating protein (GAP) and acts as a negative regulator of the Rheb/mTOR pathway.
Interaction: Interacts with TSC1, HERC1, HSPA1, HSPA8, DAPK1, FBXW5, NAA10, and RRAGA.
Biology Area: Cell Biology, Signal Transduction
Antigen References: 1. Hobbs RM, et al. 2015. EMBO Rep. 16:467. 2. Demetriades C, et al. 2014. Cell. 156:786. 3. Tripathi DN, et al. 2013. Proc. Natl. Acad. Sci. USA. 110:E2950. 4. Han JM, et al. 2011. FEBS Lett. 585:973. 5. Huang J, et al. 2008. Biochem J. 412:179. 6. Li Y, et al. 2004. Trends Biochem Sci. 29:32.
Gene ID: 7249
UniProt: View information about TSC2 on UniProt.org
Clone: W16104A
Regulatory Status: RUO
Other Names: Tuberin, Tuberous sclerosis 2 protein, TSC4
Isotype: Rat IgG2a, κ