Biolegend, 697802, Purified anti-Claudin-1 Antibody, 100μg

Claudin-1 is a member of the claudin family of proteins, which is composed of at least 24 members. Claudin proteins are integral membrane proteins with 4 transmembrane regions and 2 extracellular loops that form tight junctions. Tight junctions function as a channel and a barrier to maintain cellular morphology, polarity, and homeostasis between epithelial cells. Claudin-1 is a main component of tight junctions and is sorely responsible for normal barrier function of the skin. Misregulated expression of Claudin-1 has been shown to be associated with cancer progression and metastasis in several cancers. Claudin-1 has been found to be overexpressed in neoplasia associated with inflammatory bowel disease. Mutations in Claudin-1 gene lead to human skin diseases and Claudin-1 deficient mice die soon after birth due to dehydration.
100μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Synthetic Claudin-1 peptide conjugated to KLH (TPRPYPKPAPSSGKDYVC)
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: This clone has no cross-reactivity with mouse (in house tested).
RRID: AB_2687205 (BioLegend Cat. No. 697801) AB_2687191 (BioLegend Cat. No. 697802)
Structure: 211 amino acids with a predicted molecular weight of 22.7 kD and observed approximately 18kD. Multi-pass membrane protein with 4 transmembrane domains.
Distribution: Cell membrane. Cell junction.
Function: Claudin-1 is an integral membrane protein that forms tight junctions between skin epithelial cells to form barriers, regulate paracellular transport, and maintain cell polarity.
Interaction: Forms homopolymers and heteropolymers with other claudin family proteins. Interacts with CLDN3, TJP1/ZO-1, TJP2/ZO-2, TJP3/ZO-3, MPDZ, and PATJ.
Cell Type: Mesenchymal Stem Cells
Biology Area: Stem Cells
Antigen References: 1. Suh Y, et al. 2017. Oncogene. 36:1167. 2. Tokumasu R, et al. 2016. Proc. Natl. Acad. Sci. USA. 113:E4061. 3. Zhou B, et al. 2015. J. Clin. Med. 4:1960. 4. Saeedi BJ, et al. 2015. Mol. Biol. Cell. 26:2252. 5. Huang J, et al. 2015. Oncotarget. 6:1652. 6. Izraely S, et al. 2015. Int. J. Cancer. 136:1296.
Gene ID: 9076
UniProt: View information about Claudin-1 on UniProt.org
Clone: 9E12C72
Regulatory Status: RUO
Other Names: Senescence-associated epithelial membrane protein, CLDN1, CLD1, SEMP1,
Isotype: Mouse IgG1, κ