Biolegend, 698302, Purified anti-CRISPR (Cas9) Antibody, 100μg

Cas9 is an RNA-guided DNA endonuclease enzyme associated with the CRISPR (Clustered Regularly Interspersed Palindromic Repeats) adaptive immunity system in Streptococcus pyogenes, among other bacteria. Cas9 interrogates and cleaves foreign DNA, such as invading bacteriophage DNA or plasmid DNA. Cas9 performs this interrogation by unwinding foreign DNA and checking if it is complementary to the 20 base pair spacer region of the guide RNA. If the DNA substrate is complementary to the guide RNA, Cas9 cleaves the invading DNA. In this sense, the CRISPR-Cas9 mechanism has a number of parallels with the RNA interference (RNAi) mechanism in eukaryotes. This product was carefully developed for performance and specificity using the following products from Horizon Discovery, a Revvity company: HAP1 Cas9 Stable Cell Line (Horizon Cat No. HD Cas9-011)
100μg
Verified Reactivity: S. Pyogenes
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: C-terminal recombinant fragment of S. pyogenes Cas9
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested IP - Verified
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.05 - 0.5 µg per ml (1:1000 - 1:10,000). For immunoprecipitation, the suggested use of this reagent is 2 µg/test. It is recommended that the reagent be titrated for optimal performance for each application.
Product Citations: Farrell K, et al. 2024. Elife. :12. PubMed
RRID: AB_2715788 (BioLegend Cat. No. 698301) AB_2715782 (BioLegend Cat. No. 698302)
Structure: The architectures of Cas9 enzymes define nucleic acid binding clefts to form a central channel where DNA substrates are bound. The predicted Mw is 158kDa.
Distribution: S. Pyogenes
Function: Part of the CRISPR/Cas9 system of acquired immunity that bacteria have evolved to destroy foreign DNA elements, such as bacteriophages.
Interaction: Binds crRNA and tracrRNA
Antigen References: 1. Mali P, et al. 2013. Science 339:823.
Gene ID: 901176
UniProt: View information about CRISPR on UniProt.org
Clone: 6G12-H11
Regulatory Status: RUO
Other Names: CRISPR associated protein 9, SpyCas9
Isotype: Mouse IgG1, κ