Biolegend, 698702, Purified anti-TCF12 Antibody, 100μg

TCF12, also known as HTF4 or HEB, is a member of class I basic helix-loop-helix (bHLH) protein family that recognizes the consensus E-box (5'-CANNTG-3') DNA binding site. TCF12 is a transcription factor that participates in regulating gene expression through formation of homodimer or heterodimers with other bHLH E-proteins. TCF12 has been shown to be involved in neuron differentiation and is upregulated in certain cancer cells. TCF12 functions as a transcriptional repressor responsible for down-regulating expression of E-cadherin in colorectal cancer cells. TCF12-deficient mice show abnormality in brain development and a high percentage of postnatal death. Mutations in TCF12 gene have been linked to coronal craniosynostosis.
100μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Human TCF12 recombinant protein (108-521 a.a.) expressed in E. coli.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.2 - 2.0 µg per ml (1:250-1:2500 diluted). It is recommended that the reagent be titrated for optimal performance for each application.
RRID: AB_2687319 (BioLegend Cat. No. 698701) AB_2687320 (BioLegend Cat. No. 698702)
Structure: 706 amino acids with a predicted molecular weight of 76 kD. Contains a C-terminal bHLH domain responsible for DNA binding.
Distribution: Nucleus
Function: TCF12 is a transcriptional regulator involved in neuronal development and is up-regulated in some type of tumors.
Interaction: Forms a homodimer or a heterodimer with myogenin, E12 and ITF2 proteins. Interacts with PTF1A, NEUROD2, RUNX1T1, AML1-MTG8/ETO, and BHLHA9.
Biology Area: Cell Biology, Transcription Factors
Molecular Family: Nuclear Markers
Antigen References: 1. Yi S, et al. 2017. Stem Cells. 35:386. 2. Chen YF, et al. 2016. Cancer Res. 76:4872. 3. Goos JA, et al. 2016. Hum. Mutat. 37:732. 4. Labreche K, et al. 2015. Nat. Commun. 6:7207. 5. Sharma VP, et al. 2013. Nat. Genet. 45:304. 6. Lee CC, et al. 2012. J. Biol. Chem. 287:2798.
Gene ID: 6938
UniProt: View information about TCF12 on UniProt.org
Clone: W16168A
Regulatory Status: RUO
Other Names: Transcription factor 12, Class B basic helix-loop-helix protein 20 (bHLHb20), HEB, HTF4
Isotype: Rat IgG2b, κ