Biolegend, 699951, PhosphoPair ERK1/2 (Thr202/Tyr204) Antibody Set, 1kit

ERK1/2 are members of mitogen-activated kinases (MAPKs) of serine/threonine protein kinases. ERK1/2 can be activated by a range of extracellular stimuli, such as mitogen, growth factors, neurotransmitters, chemokines, and cytokines, through receptor tyrosine kinases (RTK), G protein-coupled receptors (GPCRs), or protein kinase C (PKC). Upon stimulation, ERK1/2 are phosphorylated by the upstream kinase MEK on residues Thr202 and Tyr204 and in turn phosphorylate many other downstream molecules that are involved in a range of cellular processes such as cell proliferation, differentiation, motility and cell death.
1kit
Verified Reactivity: Human, Mouse
Immunogen: Please refer to individual product datasheets for immunogen information
Formulation: Phosphate-buffered solutions, pH 7.2, containing 0.09% sodium azide
Preparation: The antibodies were purified by affinity chromatography.
Storage & Handling: These antibody solutions should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested
Recommended Usage: Each lot of antibodies in this set is quality control tested by Western blotting. For Western blotting, the suggested uses of these reagents are as follows: Clone W15133B: 0.5 - 2.0 µg/mL Clone 6B8B69: 0.1 - 1.0 µg/mL It is recommended that the reagent be titrated for optimal performance for each application. For recommended usage information for additional applications, please refer to the datasheet of the individual products.
Structure: ERK1 is a 379 amino acid protein with a predicted molecular weight of 43 kD. ERK2 is a 360 amino acid protein with a predicted molecular weight of 41 kD.
Distribution: Ubiquitously expressed/Nucleus and cytosol
Function: ERK1/2 are broadly expressed protein kinases that are involved in proliferation, differentiation, motility, and cell death. In response to extracellular stimuli, ERK1 and ERK2 are phosphorylated at Thr202 and Tyr204, and in turn transduce signals to downstream substrates.
Interaction: ERK1/2 phosphorylates a wide range of downstream substrates, such as ATF2, BCL6, EIF4EBP1, CANX, MAP2, DAPK1, RAF1, SYK, and GRB10.
Antigen References: 1. Futran AS, et al. 2013. Curr. Biol. 23:R972. 2. Mendoza MC, et al. 2011. Trends Biochem. Sci. 36:320. 3. Chambard JC, et al. 2007. Biochim. Biophys. Acta. 1773:1299. 4. Roux PP and Blenis J. 2004. Microbiol. Mol. Biol. Rev. 68:320. 5. Torii S, et al. 2004. J. Biochem. 136:557. 6. Clark EA and Haynes RO. 1996. J. Biol. Chem. 271:14814.
Gene ID: 55955594
UniProt: View information about ERK1/2 on UniProt.org
Clone: 6B8B69; W15133B;
Regulatory Status: RUO
Other Names: Extracellular Signal-Regulated Kinase 1, Extracellular Signal-Regulated Kinase 2, Mitogen-Activated Protein Kinase 3, MAPK3, p44, p42, p44-ERK1, p44-ERK2, p44-MAPK,, p42-MAPK