Biolegend, 714155, Recombinant Human ANGPTL-2 (carrier-free), 50μg

Angiopoietin-like Protein (ANGPTL)-2 is a member of a family of secreted glycoproteins that are structurally related to Tie-2 receptor ligand angiopoietins, but do not bind to either Tie-2 or the homologous Tie-1 receptor. ANGPTL-2 is an important adipocyte-derived mediator of chronic inflammation in obesity and in systemic insulin resistance. Its expression is increased by obesity and obesity-related pathological conditions, including hypoxia and endoplasmic reticulum (ER) stress. ANGPTL-2 act on endothelial cells and monocytes or macrophages via integrin signaling, resulting in the promotion of inflammation. Elevated expression levels of ANGPTL-2 were measured during carcinogenesis in a chemically induced skin squamous cell carcinoma model. ANGPTL-2 is among the proinflammatory factors that are overexpressed and induce Epithelial Mesenchymal Transition (EMT) in pancreatic duct adenocarcinoma cells with acquired resistance to anti-VEGF treatment. Human leukocyte immunoglobulin-like receptor B2 (LILRB2) has been identified as the receptor for ANGPTLs. Patients with lung cancer showing elevated expression of ANGPTL-2 in cells within the primary tumor were associated with a reduced disease-free survival after surgical resection.
50μg
Source: Human ANGPTL-2, amino acids Thr260-His493 (Accession # Q9UKU9.1), with a C-terminal 6xHis tag was expressed in CHO cells.
Molecular Mass: The 243 amino acid recombinant protein has a predicted molecular mass of approximately 28.5 kD. The protein migrates approximately at 28-30 kD in SDS-PAGE in DTT-reduced and non-reduced conditions.
N-terminalSequence Analysis: The predicted N-terminal amino acid is Thr.
Purity: > 95%, as determined by Coomassie stained SDS-PAGE.
Formulation: Lyophilized from 0.22 µm filtered protein solution in PBS, pH 7.2 with 5% trehalose.
Endotoxin Level: Less than 1 EU per µg of protein as determined by the LAL method
Concentration: 50 µg size, Lyophilized
Storage & Handling: Unopened vial can be stored between 2°C -4°C for up to two weeks and at -20°C or colder until the expiration date. Reconstitute lyophilized protein in sterile water preferably in sterile deionized water. Before reconstitution, make sure sterile water and the product are at room temperature. Quickly spin the vial or gently tap down on the vial to make sure the lyophilized product is at the bottom of the vial before opening. Use aseptic techniques to add the required volume of reconstitution buffer (sterile water) to the vial, to obtain the recommended stock concentration 250 μg/mL. Close the vial and leave at an ambient temperature for 15-20 minutes. Then gently invert the vial several times or until all the lyophilized product dissolves. Leave the vial at room temperature for another 15 minutes. If small particulates are still observed after 15 minutes, incubate at room temperature for an additional 30 minutes and leave the vial at 2°C - 8°C overnight. Next day, invert the vial several times or gently pipette the solution up and down before use. If needed, transfer the reconstituted stock solution to a sterile container for additional dilution to no less than 100 μg/mL. Small working aliquots in polypropylene tubes can be made after reconstitution and store the vials at -20°C or lower. Avoid freeze/thaw cycles. Carrier protein such as 0.2 - 1% endotoxin-free BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to two weeks or stored at -20°C or colder for up to 3 months.
Activity: Immobilized human ANGPTL-2 binds to biotinylated human LILRB2 in a dose-dependent manner. ED50 for this effect is 0.8-4.8 ng/mL.
Application: Bioassay
Application Notes: Our lyophilized proteins are validated in-house to maintain activity after shipping at ambient temperature and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at [email protected].
Structure: Dimer
Function: Mediator of chronic inflammation in obesity and in systemic insulin resistance, tumor suppressor in ovarian cancer
Interaction: Endothelial cells and monocytes or macrophages
Ligand/Receptor: Leukocyte immunoglobulin-like receptor B2 (LILRB2)
Bioactivity: Immobilized human ANGPTL-2 binds to biotinylated human LILRB2 in a dose-dependent manner. ED50 for this effect is 0.8-4.8 ng/mL.
Cell Type: Endothelial cells, Macrophages, Monocytes
Biology Area: Cell Biology, Signal Transduction
Molecular Family: Soluble Receptors, Tumor Suppressors
Antigen References: Tortora G, et al. 2004. Curr. Pharm. Des. 10:11-26. Kim I, et al. 1999. J Biol Chem. 274:26523-26528. Tabata M, et al. 2009. Cell metabolism. 10:178-188. Aoi J, et al. 2011. Cancer Res. 71:7502-7512 Carbone C, et al. 2011. Clin. Cancer Res. 17:5822-5832. Zheng J, et al. 2012. Nature. 485:656-660. Endo M, et al. 2012. Cancer Res. 72:1784-1794. Carbone C. et al. 2015. Oncotarget. 6:13822-13834. Kim I. et al. 1999. J. Biol Chem. 274:26523-26528.
Gene ID: 23452
UniProt: View information about ANGPTL-2 on UniProt.org
Regulatory Status: RUO
Other Names: angiopoietin-like 2, Angiopoietin-like Protein 2, angiopoietinrelated protein 2, ANGPTL2, ANGRP2
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Q: How does the activity of your recombinant proteins compare to competitors?
A: We quality control each and every lot of recombinant protein. Not only do we check its bioactivity, but we also compare it against other commercially available recombinant proteins. We make sure each recombinant protein’s activity is at least as good as or better than the competition’s. In order to provide you with the best possible product, we ensure that our testing process is rigorous and thorough. If you’re curious and eager to make the switch to BioLegend recombinants, contact your sales representative today!
Q: What is the specific activity or ED50 of my recombinant protein?
A: The specific activity range of the protein is indicated on the product datasheets. Because the exact activity values on a per unit basis can largely fluctuate depending on a number of factors, including the nature of the assay, cell density, age of cells/passage number, culture media used, and end user technique, the specific activity is best defined as a range and we guarantee the specific activity of all our lots will be within the range indicated on the datasheet. Please note this only applies to recombinants labeled for use in bioassays. ELISA standard recombinant proteins are not recommended for bioassay usage as they are not tested for these applications.
Q: Have your recombinants been tested for stability?
A: Our testing shows that the recombinant proteins are able to withstand room temperature for a week without losing activity. In addition the recombinant proteins were also found to withstand four cycles of freeze and thaw without losing activity.
Q: Does specific activity of a recombinant protein vary between lots?
A: Specific activity will vary for each lot and for the type of experiment that is done to validate it, but all passed lots will have activity within the established ED50 range for the product and we guarantee that our products will have lot-to-lot consistency. Please conduct an experiment-specific validation to find the optimal ED50 for your system.
Q: How do you convert activity as an ED50 in ng/ml to a specific activity in Units/mg?
A: Use formula Specific activity (Units/mg) = 10^6/ ED50 (ng/mL)