Biolegend, 716956, Recombinant Mouse IL-24 (carrier-free), 100μg

Interleukin-24 (IL-24), a cytokine belonging to the IL-10 family, is produced by activated monocytes, macrophages, T cells, keratinocytes, and epithelial cells. Mature mouse IL-24 shares approximately 69% amino acid sequence identity with human IL-24. IL-24 exerts its biological effects by signaling through two heterodimeric receptor complexes, IL-20R1/IL-20R2 or IL-22R1/IL-20R2, leading to the activation of the JAK/STAT, MAPK, and PI3K/AKT pathways. Functionally, IL-24 plays a critical role in immune regulation, host defense, inflammation, cell proliferation, differentiation, wound healing, and tissue remodeling. In monocytes, IL-24 induces the production of proinflammatory cytokines such as TNF-α, IL-6, and IL-1β, while in B cells, it suppresses plasma cell differentiation. IL-24 selectively induces apoptosis in various cancer cell types, including melanoma, breast, lung, prostate, and pancreatic cancers. IL-24 also suppresses angiogenesis by inhibiting endothelial cell proliferation and migration. Furthermore, IL-24 enhances the recruitment and activation of immune cells, such as NK cells and cytotoxic T lymphocytes, within the tumor microenvironment. While IL-24 contributes to host defense mechanisms, it can also promote pathological inflammation observed in conditions such as rheumatoid arthritis, psoriasis, and inflammatory bowel disease.
100μg
Source: Recombinant mouse IL-24, amino acid Gln27-Leu181 (Accession No. Q925S4) with a C-terminal GGS-8His tag was expressed in E. coli.
Molecular Mass: The 167 amino acid recombinant protein has a predicted molecular mass of approximately 19.4 kD. The DTT-reduced and non-reduced proteins migrate at approximately 19-23 kD by SDS-PAGE. The N-terminal amino acid is Met.
Purity: > 95%, as determined by Coomassie stained SDS-PAGE.
Formulation: 0.22 µm filtered protein solution is in 4mM HCl, pH 2.4
Endotoxin Level: Less than 0.1 EU per µg protein as determined by the LAL method.
Concentration: 25 µg size is bottled at 200 µg/mL. 100 µg size and larger sizes are lot-specific and bottled at the concentration indicated on the vial. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling: Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
Activity: ED50 = 1 – 12 ug/mL as measured by its ability to support the adhesion of COLO 205 cells.
Application: Bioassay
Application Notes: BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue-ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are validated in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at [email protected].
Structure: Monomer
Distribution: Activated monocytes, macrophages, Th2 cells, keratinocytes, NK cells, and epithelial cells
Function: Immune regulation, inflammation, cell proliferation, differentiation, wound healing, and tissue remodeling
Interaction: Kupffer cells, hepatocytes, endothelial cells, pancreatic acinar cells, and epithelial cell
Ligand/Receptor: IL-20R1/IL-20R2 or IL-22R1/IL-20R2
Bioactivity: Measured by its ability to support the adhesion of COLO 205 cells
Cell Type: B cells, Epithelial cells, Macrophages, Monocytes, NK cells, Th2
Biology Area: Adaptive Immunity, Angiogenesis, Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Cell Death, Immuno-Oncology, Immunology, Signal Transduction
Molecular Family: Cytokine/Chemokine Receptors, Cytokines/Chemokines, Innate Immune Signaling, Tumor Suppressors
Antigen References: Chen J, et al. 2023. iScience. 26(9):107531. Van Belle AB, et al. 2019. Sci Rep. 9(1):1852. Panneerselvam J, et al. 2002. Cancers. 11(12):1879. Sie C, et al. 2022. J Exp Med. 219(8): e20212443. Chong WP, et al. 2020. Immunity. 53(2): 384. Ramesh R, et al. 2009. Cancer Res. 63(30):7783. Parrish-Novak J, et al. 2002. J Biol Chem. 277(49):47517. Maarof, G, et al. 2010. Blood. 115:1718. Caudell, EG, et al. 2002. J. Immunol. 168: 6041.
Gene ID: 93672
UniProt: View information about IL-24 on UniProt.org
Regulatory Status: RUO
Other Names: Interleukin 24, IL24, IL-24, IL10B, FISP, C49A, melanocyte associated Mda-7, MDA-7, MDA7MOB5, melanoma differentiation association protein 7
Q: Why choose BioLegend recombinant proteins?
A: • Each lot of product is quality-tested for bioactivity as indicated on the data sheet.
     • Greater than 95% Purity or higher, tested on every lot of product.
     • 100% Satisfaction Guarantee for quality performance, stability, and consistency.
     • Ready-to-use liquid format saves time and reduces challenges associated with reconstitution.
     • Bulk and customization available. Contact us.
     • Learn more about our Recombinant Proteins.
Q: How does the activity of your recombinant proteins compare to competitors?
A: We quality control each and every lot of recombinant protein. Not only do we check its bioactivity, but we also compare it against other commercially available recombinant proteins. We make sure each recombinant protein’s activity is at least as good as or better than the competition’s. In order to provide you with the best possible product, we ensure that our testing process is rigorous and thorough. If you’re curious and eager to make the switch to BioLegend recombinants, contact your sales representative today!
Q: What is the specific activity or ED50 of my recombinant protein?
A: The specific activity range of the protein is indicated on the product datasheets. Because the exact activity values on a per unit basis can largely fluctuate depending on a number of factors, including the nature of the assay, cell density, age of cells/passage number, culture media used, and end user technique, the specific activity is best defined as a range and we guarantee the specific activity of all our lots will be within the range indicated on the datasheet. Please note this only applies to recombinants labeled for use in bioassays. ELISA standard recombinant proteins are not recommended for bioassay usage as they are not tested for these applications.
Q: Have your recombinants been tested for stability?
A: Our testing shows that the recombinant proteins are able to withstand room temperature for a week without losing activity. In addition the recombinant proteins were also found to withstand four cycles of freeze and thaw without losing activity.
Q: Does specific activity of a recombinant protein vary between lots?
A: Specific activity will vary for each lot and for the type of experiment that is done to validate it, but all passed lots will have activity within the established ED50 range for the product and we guarantee that our products will have lot-to-lot consistency. Please conduct an experiment-specific validation to find the optimal ED50 for your system.
Q: How do you convert activity as an ED50 in ng/ml to a specific activity in Units/mg?
A: Use formula Specific activity (Units/mg) = 10^6/ ED50 (ng/mL)