Biolegend, 740777, LEGENDplex™ Human Inflammation Panel 2 Detection Antibodies, VO24

The LEGENDplex™ Human Inflammation Panel 2 Detection Antibodies contains 13 anti-human antibodiesa against Free Active TGF-b1, PAI-1, sTREM-1, PTX3, sCD40L, sCD25 (IL-2Ra), CXCL12 (SDF-1), sST2, sTNF-RI, sTNF-RII, sRAGE, CX3CL1 (Fractalkine), sCD130 (gp130). This Detection cocktail is intended for use with the following in Mix and Match assays: Human Inflammation Panel 2 740778 LEGENDplex™ Human Inflammation Panel 2 Standard 740779 LEGENDplex™ Human Free Active TGF-b1 Capture Bead A4, 13X 740780 LEGENDplex™ Human PAI-1 Capture Bead A5, 13X 740781 LEGENDplex™ Human sTREM-1 Capture Bead A6, 13X 740782 LEGENDplex™ Human PTX3 Capture Bead A7, 13X 740783 LEGENDplex™ Human sCD40L Capture Bead A8, 13X 740784 LEGENDplex™ Human sCD25 (IL-2Ra) Capture Bead A10, 13X 740785 LEGENDplex™ Human CXCL12 (SDF-1) Capture Bead B2, 13X 740786 LEGENDplex™ Human sST2 Capture Bead B3, 13X 740787 LEGENDplex™ Human sTNF-RI Capture Bead B4, 13X 740788 LEGENDplex™ Human sTNF-RII Capture Bead B5, 13X 740789 LEGENDplex™ Human sRAGE Capture Bead B6, 13X 740790 LEGENDplex™ Human CX3CL1 (Fractalkine) Capture Bead B7, 13X 740791 LEGENDplex™ Human sCD130 (gp130) Capture Bead B9, 13X 741206 LEGENDplex™ Buffer Set H1 740377 LEGENDplex™ Filter Plate OR 740379 LEGENDplex™ V-Bottom Plate
VO24
Verified Reactivity: Human
Application: Multiplex Learn more about LEGENDplex™ at biolegend.com/legendplex Download the LEGENDplex™ software here.
RRID: SCR_001134 (BioLegend Cat. No. 740777)
Biology Area: Cell Biology, Innate Immunity, Neuroinflammation, Neuroscience
Molecular Family: Cytokines/Chemokines
Gene ID: NA
Regulatory Status: RUO
Other Names: Free Active TGF-b1, PAI-1, sTREM-1, PTX3, sCD40L, sCD25 (IL-2Ra), CXCL12 (SDF-1), sST2, sTNF-RI, sTNF-RII, sRAGE, CX3CL1 (Fractalkine), sCD130 (gp130)
Q: If I don't have a vacuum, how do I remove the liquid from my plate?
A: If you do not have a vacuum, the assay should be run in a V-bottom plate. After centrifugation using a swinging-bucket rotor with a plate adaptor, you can remove the liquid by flicking the plate quickly, dumping the contents into a sink, and patting it dry carefully on a stack of clean paper towels without losing the beads. Alternatively, you can remove the liquid by using a pipette.
Q: Should I perform the assay with the filter plates or with V-bottom plates?
A: Filter plates or V-bottom plates have been included in some kits for your convenience. A vacuum filtration unit is required to work with the filter plates. However, if you don’t have access to a vacuum manifold or if you prefer, then you can use the V-bottom plates and follow the recommended assay protocols for the type of plates you choose. All plates should be made from low binding polypropylene. Polystyrene ELISA or cell culture plates should not be used.
Q: After I finish the staining process, how long can I wait before reading my LEGENDplex™ samples?
A: The samples can be kept overnight at 4°C while being protected from exposure to light and be read the next day. There may be a decrease in signal, but overall, the assay results should not be affected. Storing the samples for extended periods of time is not recommended, as it could lead to further reductions in signal.
Q: What is the shelf life of LEGENDplex™ kits?
A: LEGENDplex™ kits are guaranteed for 6 months from the date of receipt, but may have a shelf life of up to 2 years from the date of manufacture.
Q: Is special software required for data analysis?
A: Typically flow cytometers generate output files in FCS format (e.g. FCS 2.0, 3.0, or 3.1) and in some cases in list mode file format (LMD). Other software may be available to analyze FCS files. Data generated using LEGENDplex™ kits can be analyzed using the freely available LEGENDplex™ data analysis software. Please check our website for the most updated versions of the software.