Biolegend, 740852, LEGENDplex™ Mouse Macrophage/Microglia Panel Standard, 1VIAL

The LEGENDplex™ Mouse Macrophage/Microglia Panel Standard contains 13 mouse proteins, including CXCL1 (KC), Free Active TGF-β1, IL-18, IL-23, CCL22 (MDC), IL-10, IL-12p70, IL-6, TNF-α, G-CSF, CCL17 (TARC), IL-12p40, and IL-1β. The beads are intended for use with the following in Mix and Match assays: Mouse Macrophage/Microglia Panel 740851 LEGENDplex™ Mouse Macrophage/Microglia Panel Detection Antibodies 740853 LEGENDplex™ Mouse CXCL1 (KC) Capture Bead A4, 13X 740854 LEGENDplex™ Mouse Free Active TGF-β1 Capture Bead A5, 13X 740855 LEGENDplex™ Mouse IL-18 Capture Bead A6, 13X 740856 LEGENDplex™ Mouse IL-23 Capture Bead A7, 13X 740857 LEGENDplex™ Mouse CCL22 (MDC) Capture Bead A8, 13X 740858 LEGENDplex™ Mouse IL-10 Capture Bead A10, 13X 740859 LEGENDplex™ Mouse IL-12p70 Capture Bead B2, 13X 740860 LEGENDplex™ Mouse IL-6 Capture Bead B3, 13X 740861 LEGENDplex™ Mouse TNF-α Capture Bead B4, 13X 740862 LEGENDplex™ Mouse G-CSF Capture Bead B5, 13X 740863 LEGENDplex™ Mouse CCL17 (TARC) Capture Bead B6, 13X 740864 LEGENDplex™ Mouse IL-12p40 Capture Bead B7, 13X 740865 LEGENDplex™ Mouse IL-1β Capture Bead B9, 13X 741446 LEGENDplex™ Buffer Set B1 740377 LEGENDplex™ Filter Plate OR 740379 LEGENDplex™ V-Bottom Plate
1VIAL
Verified Reactivity: Mouse
Application: Multiplex Learn more about LEGENDplex™ at biolegend.com/legendplex Download the LEGENDplex™ software here.
Product Citations: Meleady L, et al. 2023. Epigenetics. 2241008:18. PubMed Barbosa-Matos C, et al. 2024. Respir Res. 257:25. PubMed
Biology Area: Immunology, Innate Immunity, Neuroinflammation, Neuroscience
Molecular Family: Cytokines/Chemokines
Gene ID: NA
Regulatory Status: RUO
Other Names: CXCL1 (KC), Free Active TGF-β1, IL-18, IL-23, CCL22 (MDC), IL-10, IL-12p70, IL-6, TNF-α, G-CSF, CCL17 (TARC), IL-12p40, IL-1β
Q: If I don't have a vacuum, how do I remove the liquid from my plate?
A: If you do not have a vacuum, the assay should be run in a V-bottom plate. After centrifugation using a swinging-bucket rotor with a plate adaptor, you can remove the liquid by flicking the plate quickly, dumping the contents into a sink, and patting it dry carefully on a stack of clean paper towels without losing the beads. Alternatively, you can remove the liquid by using a pipette.
Q: Should I perform the assay with the filter plates or with V-bottom plates?
A: Filter plates or V-bottom plates have been included in some kits for your convenience. A vacuum filtration unit is required to work with the filter plates. However, if you don’t have access to a vacuum manifold or if you prefer, then you can use the V-bottom plates and follow the recommended assay protocols for the type of plates you choose. All plates should be made from low binding polypropylene. Polystyrene ELISA or cell culture plates should not be used.
Q: After I finish the staining process, how long can I wait before reading my LEGENDplex™ samples?
A: The samples can be kept overnight at 4°C while being protected from exposure to light and be read the next day. There may be a decrease in signal, but overall, the assay results should not be affected. Storing the samples for extended periods of time is not recommended, as it could lead to further reductions in signal.
Q: What is the shelf life of LEGENDplex™ kits?
A: LEGENDplex™ kits are guaranteed for 6 months from the date of receipt, but may have a shelf life of up to 2 years from the date of manufacture.
Q: Is special software required for data analysis?
A: Typically flow cytometers generate output files in FCS format (e.g. FCS 2.0, 3.0, or 3.1) and in some cases in list mode file format (LMD). Other software may be available to analyze FCS files. Data generated using LEGENDplex™ kits can be analyzed using the freely available LEGENDplex™ data analysis software. Please check our website for the most updated versions of the software.