Biolegend, 741333, LEGENDplex™ SARS-CoV-2 Variants Neut. Ab Panel (6-x) Detection, VO24

The LegendPlex™ SARS-CoV-2 Variants Neutralization Antibody Panel (6-plex) Mix and Match Beads provide beads for 6 targets including Variant Alpha, Variant Beta, Variant Omicron, Variant Gamma, Wild type S1 protein and Variant Delta. These beads are recommended for use with each other and the following in a Mix and Match Assay. Biotinylated ACE2 protein is serving as the Detection in the assay. The detection and the standard neutralization antibody or samples will compete to bind to the capture beads. Therefore, the assay displays an inverted standard curve and the amount of analyte will be negatively correlated to the signal intensities. 741324 LEGENDplex™ SARS-CoV-2 S1 Variant Alpha Bead B2, 13X 741325 LEGENDplex™ SARS-CoV-2 S1 Variant Beta Bead B3, 13X 741326 LEGENDplex™ SARS-CoV-2 S1 Variant Omicron Bead B5, 13X 741327 LEGENDplex™ SARS-CoV-2 S1 Variant Gamma Bead B6 13X 741328 LEGENDplex™ SARS-CoV-2 S1 Wild Type Bead B7, 13X 741329 LEGENDplex™ SARS-CoV-2 S1 Variant Delta Bead B9, 13X 741330 LEGENDplex™ SARS-CoV-2 Variants Neut. Ab Panel (6-x) Standard 740374 LEGENDplex™ Buffer Set C 740377 LEGENDplex™ Filter Plate OR 740379 LEGENDplex™ V-bottom Plate
VO24
Verified Reactivity: Human
Application: Multiplex Learn more about LEGENDplex™ at biolegend.com/legendplex Download the LEGENDplex™ software here.
Gene ID: 43740568
Regulatory Status: RUO
Other Names: Neutralizing antibodies against Spike S1 protein of SARS-CoV-2 Variants: Alpha (B.1.1.7), Beta (B.1.351), Gamma (P.1), Delta(B.1.617.2), Omicron (B.1.1.529) and Wild Type S1 protein
Q: If I don't have a vacuum, how do I remove the liquid from my plate?
A: If you do not have a vacuum, the assay should be run in a V-bottom plate. After centrifugation using a swinging-bucket rotor with a plate adaptor, you can remove the liquid by flicking the plate quickly, dumping the contents into a sink, and patting it dry carefully on a stack of clean paper towels without losing the beads. Alternatively, you can remove the liquid by using a pipette.
Q: Should I perform the assay with the filter plates or with V-bottom plates?
A: Filter plates or V-bottom plates have been included in some kits for your convenience. A vacuum filtration unit is required to work with the filter plates. However, if you don’t have access to a vacuum manifold or if you prefer, then you can use the V-bottom plates and follow the recommended assay protocols for the type of plates you choose. All plates should be made from low binding polypropylene. Polystyrene ELISA or cell culture plates should not be used.
Q: After I finish the staining process, how long can I wait before reading my LEGENDplex™ samples?
A: The samples can be kept overnight at 4°C while being protected from exposure to light and be read the next day. There may be a decrease in signal, but overall, the assay results should not be affected. Storing the samples for extended periods of time is not recommended, as it could lead to further reductions in signal.
Q: What is the shelf life of LEGENDplex™ kits?
A: LEGENDplex™ kits are guaranteed for 6 months from the date of receipt, but may have a shelf life of up to 2 years from the date of manufacture.
Q: Is special software required for data analysis?
A: Typically flow cytometers generate output files in FCS format (e.g. FCS 2.0, 3.0, or 3.1) and in some cases in list mode file format (LMD). Other software may be available to analyze FCS files. Data generated using LEGENDplex™ kits can be analyzed using the freely available LEGENDplex™ data analysis software. Please check our website for the most updated versions of the software.