Biolegend, 741510, LEGENDplex™ Human HPV Serological IgG Panel (2-plex) w/VbP, 100tests

Human papillomavirus (HPV) is a small, non-enveloped deoxyribonucleic acid (DNA) virus that infects skin or mucosal cells. HPV16 and HPV18 are classified as high-risk oncogenic strains, predominantly implicated in the pathogenesis of HPV-associated malignancies. HPV16 is the most prevalent high-risk type, responsible for approximately 50% of cervical carcinomas, while HPV18 contributes to about 20% of cervical cancer cases. HPVs are transmitted sexually and exhibit varying prevalence rates influenced by sexual behaviors, vaccination coverage, and screening practices. Globally, the burden of HPV infection remains substantial, with HPV 16 and 18 representing key targets in cancer prevention strategies. Serological IgG assays for HPV16 and 18 are instrumental in epidemiological research and vaccine efficacy studies. The LEGENDplex™ Human HPV Serological IgG Panel is a bead-based multiplex assay, utilizing fluorescence–encoded beads suitable for use on various flow cytometers. This panel allows simultaneous quantification of 2 human antibodies, including IgG antibodies against HPV16 and HPV18. This panel provides high sensitivities and a broad dynamic range. The panel has been validated for use on serum and plasma samples.
100tests
Verified Reactivity: Human
Application: Multiplex - Panel Learn more about LEGENDplex™ at biolegend.com/legendplex Download the LEGENDplex™ software here.
Molecular Family: Blood Group Markers
Gene ID: 14890821489090
Regulatory Status: RUO
Other Names: Human papillomavirus type 16, Human papillomavirus type 18, Genital human papillomavirus, High risk HPV
Q: If I don't have a vacuum, how do I remove the liquid from my plate?
A: If you do not have a vacuum, the assay should be run in a V-bottom plate. After centrifugation using a swinging-bucket rotor with a plate adaptor, you can remove the liquid by flicking the plate quickly, dumping the contents into a sink, and patting it dry carefully on a stack of clean paper towels without losing the beads. Alternatively, you can remove the liquid by using a pipette.
Q: Should I perform the assay with the filter plates or with V-bottom plates?
A: Filter plates or V-bottom plates have been included in some kits for your convenience. A vacuum filtration unit is required to work with the filter plates. However, if you don’t have access to a vacuum manifold or if you prefer, then you can use the V-bottom plates and follow the recommended assay protocols for the type of plates you choose. All plates should be made from low binding polypropylene. Polystyrene ELISA or cell culture plates should not be used.
Q: After I finish the staining process, how long can I wait before reading my LEGENDplex™ samples?
A: The samples can be kept overnight at 4°C while being protected from exposure to light and be read the next day. There may be a decrease in signal, but overall, the assay results should not be affected. Storing the samples for extended periods of time is not recommended, as it could lead to further reductions in signal.
Q: What is the shelf life of LEGENDplex™ kits?
A: LEGENDplex™ kits are guaranteed for 6 months from the date of receipt, but may have a shelf life of up to 2 years from the date of manufacture.
Q: Is special software required for data analysis?
A: Typically flow cytometers generate output files in FCS format (e.g. FCS 2.0, 3.0, or 3.1) and in some cases in list mode file format (LMD). Other software may be available to analyze FCS files. Data generated using LEGENDplex™ kits can be analyzed using the freely available LEGENDplex™ data analysis software. Please check our website for the most updated versions of the software.