Biolegend, 772614, Biotinylated Recombinant Human TNFRSF9 (4-1BB)-Fc Chimera (carrier-free), 25μg

TNFRSF9, also known as 4-1BB and CD137, is a member of the tumor necrosis factor receptor superfamily (TNFRSF). It is a costimulatory receptor similar to OX40, CD27, CD30, and CD40. The interaction of TNFRSF9 and its ligand, TNFSF9, leads to the activation of cytotoxic T cells, reactivation of anergic T lymphocytes, and induces T-cell proliferation. TNFRSF9/TNFSF9 preferentially enhances the expansion of CD8+ T cells rather than CD4+ T cells, through the increase of IL-2Rα (CD25) and IL-2 expressions in CD8+ T cells. Among the TNFRSF members, TNFRSF9 is the most potent inducers of IL-2Rα expression on CD8+ T cells. In vivo studies with TNFSF9 knockout mice and with the agonistic anti-TNFRSF9 monoclonal antibody showed that TNFRSF9/TNFSF9 interaction keeps optimal CD8 T-cell mediated immune response against viral infections and tumors. The mouse TNFRSF9 has 60% identity to the human receptor at the amino acid level.
25μg
Source: Human TNFRSF9, amino acid Leu24-Gln186 (Accession # Q07011.1), with a linker (SSLGS), a C-terminal human IgG1 (Pro100-Lys330) and an Avi-tag, was expressed in 293E cells. Human TNFRSF9-Fc Chimera was site-specifically biotinylated by enzyme BirA.
Molecular Mass: The 414 amino acid recombinant protein has a predicted molecular mass of approximately 45.46 kD. The DTT-reduced and non-reduced protein migrates at approximately 62 kD and 115 kD by SDS-PAGE, respectively. The predicted N-terminal amino acid is Leu.
Purity: > 95%, as determined by Coomassie stained SDS-PAGE
Formulation: 0.22 µm filtered protein solution is in PBS, 5% Glycerol
Endotoxin Level: Less than 0.1 EU per µg protein as determined by the LAL method
Concentration: 25 µg size is bottled at 200 µg/mL. 100 µg size and larger sizes are lot-specific and bottled at the concentration indicated on the vial. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling: Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
Activity: When recombinant human TNFSF9 (Cat. No. 750002) is immobilized at 1 μg/mL, biotinylated recombinant human TNFRSF9-Fc chimera binds in a dose-dependent manner with EC50 range of 0.75 – 3.0 ng/mL. HRP Avidin (Cat. No. 405103) was used to detect the binding.
Application: Bioassay
Application Notes: BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue-ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are validated in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at [email protected].
Structure: Disulfide linked homodimer
Distribution: Activated T cells, natural killer cells, macrophages, neutrophils, dendritic cells, eosinophils, Tregs, activated B cells, mast cells, and endothelial cells in tumor capillaries
Function: Proliferation of T cells; activates their effector functions, survival, and establishes immunological memory.
Interaction: Activated antigen presenting cells, macrophages, B cells, B cell lymphoma, dendritic cells, a small fraction of T cells, epithelial, and endothelial cells
Ligand/Receptor: TNFSF9 and CD137L
Bioactivity: Measured by its ability to bind recombinant human TNFSF9.
Cell Type: B cells, Dendritic cells, Macrophages, Neutrophils, NK cells, T cells
Biology Area: Immuno-Oncology, Immunology
Molecular Family: Immune Checkpoint Receptors, Soluble Receptors
Antigen References: Pollok KE, et al. 1994. Eur J Immunol. 24:367-74. Watts TH. 2005. Annu Rev Immunol. 23:23-68. Zhu Y, et al. 2007. Blood. 109:4882-9. Locatelli F. 2009. Blood. 11:2208-9. Wang J, et al. 2010. J Immunol. 185:7654-62. Eun SY, et al. 2015. J Immunol. 1:134-41. Spencer AJ, et al. 2014. PLoS One. 9:e105520. Yi L, et al. 2014. PLoS One. 1:e86337
Gene ID: 3604
UniProt: View information about TNFRSF9 on UniProt.org
Regulatory Status: RUO
Other Names: CD137, CDw137, 4-1BB, 4-1BB ligand receptor, T cell antigen ILA, T cell antigen 4-1BB homolog