Biolegend, 795615, Cell-Vive™ GMP Recombinant Human BMP-4 (carrier-free), 50μg

Bone morphogenetic proteins (BMPs) are multi-functional growth factors that belong to the transforming growth factor beta (TGFbeta) superfamily. BMPs play a key role in embryonic development, especially during heart, neural and cartilage development. Around 20 BMP family members have been identified and characterized. BMPs signal through serine/threonine kinase receptors, composed of type I and II subtypes. Four type I receptors have been identified: type IA and IB BMP receptors, type IA activin receptor, and activin receptor-like kinase I. Three type II receptors have also been recognized: type II BMP receptor and type II / IIB activin receptors. The BMP-4 gene is conserved across human, chimpanzee, Rhesus monkey, dog, cow, mouse, rat, and zebrafish species. BMP-2 and BMP-4 are highly similar, with a 92% amino acid sequence identity. BMP-4 is required for mesoderm formation and patterning in mice, and BMP-4 KO mice are embryonic-lethal. BMP-4 has an important role in postnatal tooth cytodifferentiation. Overexpression of BMP-4 has been associated with fibrodysplasia ossificans progressiva (FOP). Overexpression of BMP-4 has also been associated with cancer, including melanoma, hepatocellular carcinoma, adenocarcinoma, and colorectal cancer cell lines. This overexpression can promote migratory and invasive properties of cancer cells. BioLegend Cell-Vive™ GMP Recombinant proteins are manufactured and tested in accordance with USP Chapter 1043, Ancillary Materials for Cell, Gene and Tissue-Engineered Products and Ph. Eur. Chapter 5.2.12 in a dedicated GMP facility compliant with ISO 13485:2016. Specifications and processes include:
50μg
Source: Human BMP-4, amino acid (Ser293-Arg408) (Accession: P12644), was expressed in CHO cells.
Molecular Mass: The 116 amino acid recombinant protein has a predicted molecular mass of approximately 13 kD. The DTT-reduced and non-reduced protein migrates at approximately 20 kD and 40 kD by SDS-PAGE, respectively. The predicted N-terminal amino acid is Ser.
N-terminalSequence Analysis: Ser-Pro-Lys-His-His-Ser-Gln-Arg-Ala-Arg
Purity: ≥ 95%, as determined by Coomassie stained SDS-PAGE
Formulation: Protein was lyophilized from 0.22 μm filtered solution containing 0.1% TFA, 10% Acetonitrile, and 5% Trehalose
Endotoxin Level: Less than or equal to 0.1 EU per μg of protein as determined by LAL method.
Residual Host Cell Protein Content: ≤ 0.500 ng/μg by ELISA
Concentration: 50 µg and 500 µg sizes are lyophilized
Storage & Handling: Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C or colder until the expiration date. Reconstitute lyophilized protein in sterile 4 mM HCl. Before reconstitution, make sure sterile 4 mM HCl and product are at room temperature. Quickly spin the vial or gently tap down on the vial to make sure the lyophilized product is at the bottom of the vial before opening. Use aseptic techniques to add the required volume of reconstitution buffer (4 mM HCl) to the vial, to obtain the recommended stock concentration 250 µg/mL. Close the vial and leave at ambient temperature for 15-20 minutes. Then gently invert the vial several times or until all the lyophilized product dissolves. Leave the vial at room temperature for another 15 minutes. If small particulates are still observed after 15 minutes, incubate at room temperature for an additional 30 minutes and leave the vial at 2°C – 8°C overnight. Next day, invert the vial several times or gently pipette the solution up and down before use. If needed, transfer the reconstituted stock solution to a sterile container for additional dilution to no less than 100 µg/mL. Small working aliquots in polypropylene tubes can be made after reconstitution and store the vials at -20°C or lower. Avoid freeze/thaw cycles. Carrier protein such as 0.2 - 1% endotoxin-free BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to two weeks or stored at -20°C or colder for up to 3 months.
Activity: BMP-4 induces alkaline phosphatase production in the chondrogenic cell line ATDC5. The ED50 for this effect is 3– 18 ng/mL.
Application: Bioassay
Application Notes: Our lyophilized proteins are validated in-house to maintain activity after shipping at ambient temperature and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at [email protected].
Application References(PubMed link indicates BioLegend citation): Zhong Z, et al. 2022. Bio Protoc. 12:24. PubMed Mao QQ, et al. 2024. Exp Ther Med. 74:27. PubMed Chen WJ, et al. 2024. Cell Death Discov. 468:10. PubMed
Disclaimer: BioLegend Cell-Vive™ GMP Recombinant proteins are for research use only. Suitable for ex vivo cell processing. Not for injection or diagnostic or therapeutic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.
Structure: Disulfide-linked homodimer
Distribution: Osteoblast, odontoblast, ameloblasts, megakaryocytes, endothelial cells. Lymphoblastoid cell lines from patients with fibrodysplasia ossificans progressiva.
Function: Neural Stem Cells, mesenchymal stem cells, hematopoietic stem and progenitors, embryonic stem cells
Interaction: Condroblast, osteoblast, retinoblastoma cells
Ligand/Receptor: Type I receptors BMPR-IA (ALK-3) and BMPR-IB (ALK-6)
Bioactivity: BMP-4 induces alkaline phosphatase production in the chondrogenic cell line ATDC5
Gene ID: 652
UniProt: View information about BMP-4 on UniProt.org
Other Names: Bone Morphogenetic Protein 4, Bone Morphogenetic Protein 2B, BMP4, BMP2B, BMP2B1