Biolegend, 800702, Purified anti-β-Amyloid, 17-24 Antibody (Previously Covance catalog# SIG-39220), 500microl

Alzheimer’s disease is characterized by the accumulation of aggregated Aβ peptides in senile plaques and vascular deposits. Aβ peptides are derived from amyloid precursor proteins (APP) through sequential proteolytic cleavage of APP by β-secretases and γ-secretases generating diverse Aβ species. Aβ can aggregate to form soluble oligomeric species and insoluble fibrillar or amorphous assemblies. Some forms of the aggregated peptides are toxic to neurons.
500microl
Verified Reactivity: Human, Mouse
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: Phosphate-buffered solution (no preservatives or carrier proteins).
Preparation: The antibody was purified by affinity chromatography.
Concentration: 1 mg/ml
Storage & Handling: This antibody should be handled aseptically as it is free of preservatives such as Sodium Azide. Store this antibody undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check the vial label or CoA to find the proper storage conditions.
Application: IHC-P - Quality tested Direct ELISA - Verified IHC-F, ICC, IP - Reported in the literature, not verified in house
Recommended Usage: Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 1.0 - 2.0 µg/ml is suggested. For Direct ELISA applications, a concentration range of 0.15 - 0.5 µg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: This antibody is reactive to amino acid residues 17-24 of ß amyloid. The epitope lies within amino acids 18-22 of ß amyloid (VFFAE). 4G8 ß-amyloid antibody reacts to abnormally processed isoforms, as well as precursor forms. This antibody clone has been reported for use on IHC of free-floating sections in PBS containing 1% Triton incubated with 0.1 m citrate buffer (4). Additional reported applications (for the relevant formats) include: immunohistochemical staining on frozen tissue sections (IHC-F) and immunocytochemistry (ICC)
Additional Product Notes: View more applications data for this product in our Scientific Poster Library.
Application References(PubMed link indicates BioLegend citation): Poduslo JF, et al. 2004. Biochem. 43:6064. (IHC-F) PubMed Forny-Germano L, et al. 2014. J Neurosci. 34:13629. (IHC-Other) PubMed Vallino Costassa E, et al. 2016. J Alzheimers Dis. 51: 875-87. (IHC-P) PubMed Chen X, et al. 2013. Neurobiol Aging. 34:2370. (ICC) PubMed Hatami A, et al. 2016. J Alzheimers Dis. 50:517. (IHC-P) PubMed Kawarabayashi T, et al. 2001. J Neurosci 21:372 (IP) Fonte V, et al. 2002. PNAS. 110:4853 (IP)
Product Citations: Jovic M, et al. 2019. PLoS One. 14:e0216726. PubMed Bishay J, et al. 2022. Sci Rep. 12:15287. PubMed Libard S, et al. 2022. J Alzheimers Dis. 90:1601. PubMed Williams D, et al. 2023. Sci Rep. 13:2337. PubMed Natarajan C, et al. 2023. Int J Mol Sci. 24:. PubMed Campbell NB, et al. 2023. Int J Mol Sci. 24:. PubMed Lia A, et al. 2023. Nat Commun. 14:1590. PubMed Jiang Y, et al. 2022. Nat Aging. 2:616. PubMed Woelfle S, et al. 2023. BMC Biol. 21:113. PubMed Illouz T, et al. 2021. Vaccine. 39:4817. PubMed Schober R, et al. 2021. Neuropathology. 41:366. PubMed Abrahamson EE, et al. 2022. Brain. 145:2161. PubMed Wirianto M, et al. 2022. FASEB J. 36:e22186. PubMed Yeap J, et al. 2022. Brain Neurosci Adv. 6:23982128221086464. PubMed Melikyan ZA, et al. 2022. Neurobiol Aging. 116:12. PubMed Feilen LP, et al. 2022. Elife. 11: . PubMed Yu WS, et al. 2022. Ann N Y Acad Sci. 1515:249. PubMed Wang Q, et al. 2022. Brain. 145:4474. PubMed Lucot KL, et al. 2022. Comp Med. 72:267. PubMed Grochowska KM, et al. 2023. EMBO J. 42:e112453. PubMed Rudan Njavro J, et al. 2022. Cells. 12: . PubMed De Schepper S, et al. 2023. Nat Neurosci. 26:406. PubMed Moon HJ, et al. 2022. Neurobiol Dis. 164:105631. PubMed Dahl MJ, et al. 2022. Neurobiol Aging. 112:39. PubMed Xiong J, et al. 2022. Nature. 603:470. PubMed Kretner B, et al. 2016. EMBO Mol Med. 10.15252/emmm.201505952. PubMed Lutzenberger M, et al. 2015. PLoS One. 10: 0134228. PubMed Crivelli SM, et al. 2021. Alzheimers Res Ther. 13:45. PubMed Forny-Germano L, et al. 2014. J Neurosci. 34:13629-13643. PubMed Doustar J, et al. 2020. Aging Cell. :e13246. PubMed Finke JM, et al. 2017. Biochim Biophys Acta. 1861:2228. PubMed A Kimura, S Hata, T Suzuki 2016. J Biol Chem. 291: 24041 - 24053. PubMed Keable A, et al. 2016. Biochim Biophys Acta. 1862:1037-1046. PubMed Sun F, et al. 2020. Front Cell Dev Biol. 8:565020. PubMed Iacono D, et al. 2020. J Neuropathol Exp Neurol. 79:144. PubMed Jäkel L, et al. 2019. Acta Neuropathol Commun. 7:141. PubMed Braak H, et al. 2018. Cereb Cortex. 28:3372. PubMed Lisa Dolfe et al. 2018. Journal of Alzheimer's disease reports. 2(1):27-39 . PubMed González C, et al. 2018. Mol Psychiatry. 23:2363. PubMed Smith AM, et al. 2021. Acta Neuropathol. Online ahead of print. PubMed Cao KJ, et al. 2021. Transl Vis Sci Technol. 10:5. PubMed Chiang ACA, et al. 2018. Am J Pathol. 188:739. PubMed Zhang L, et al. 2018. Neurobiol Dis. 114:01:00. PubMed Trambauer J, et al. 2020. EMBO Rep. 21:e47996. PubMed Elmer BM, et al. 2019. PLoS One. 14:e0226245. PubMed Wu C, et al. 2020. Med Sci Sports Exerc. 52:1456. PubMed Guo X, et al. 2020. Aging Dis. 1.446527778. PubMed Rush T, et al. 2018. J Neurosci. 38:10349. PubMed Osborne C, et al. 2018. J Cell Sci. 131:. PubMed Kim E, et al. 2021. Biomolecules. 11:. PubMed Wang C, et al. 2022. Front Pharmacol. 12:763866. PubMed Liu L, et al. 2017. Sci Rep. 7:40467. PubMed Mo D, et al. 2020. Cells. 9:00. PubMed Colvin BA, et al. 2017. J Neurochem. 143:736. PubMed Guan Z, et al. 2020. Front Cell Neurosci. 0.763888889. PubMed Uhlmann RE, et al. 2020. Nat Neurosci. 23:1580. PubMed Benbow SJ, et al. 2020. Hum Mol Genet. 495:29. PubMed Zhang H, et al. 2021. Protein Cell. 12:695. PubMed Nuvolone M, et al. 2017. PLoS One. 12:e0171923. PubMed Romani M, et al. 2021. Cell Reports. 34(3):108660. PubMed Fenyi A, et al. 2021. Cells. 10:. PubMed Yamamoto S, et al. 2021. npj Science of Food. 5(1):1. PubMed Shi H, et al. 2020. Acta Neuropathol. 139:813. PubMed Furukawa F, et al. 2018. Prion. 12:315. PubMed Wang ZH, et al. 2021. Prog Neurobiol. 202:102032. PubMed Sherman M, et al. 2016. J Neurosci. 36: 9647 - 9658. PubMed Natunen T, et al. 2020. Mol Neurodegener. 0.670833333. PubMed Wu Y, et al. 2019. Nat Commun. 10:58. PubMed Lauretti E, et al. 2017. Ann Clin Transl Neurol. 4:564. PubMed Puzzo D, et al. 2020. Journal of Clinical Investigation. 130(9):4831-4844. PubMed Kim E, et al. 2021. Sci Rep. 11:12419. PubMed Mol MO, et al. 2021. Neurol Genet. 7:e596. PubMed Cacciottolo M, et al. 2017. Transl Psychiatry. 7:e1022. PubMed Lian H, et al. 2016. J Neurosci. 36: 577 - 589. PubMed Garcia–Esparcia P, et al. 2017. Front Neurol. 8:89. PubMed Eimer WA et al. 2018. Neuron. 99(1):56-63 . PubMed Lee JH, et al. 2022. Nat Neurosci. 25:688. PubMed Zhang J, et al. 2019. Aging Cell. 18:e12978. PubMed Li JG, et al. 2019. Mol Psychiatry. 10.1038/s41380-019-0364-x. PubMed Plá V, et al. 2017. Front Mol Neurosci. 10:202. PubMed Grant MKO, et al. 2019. PLoS One. 14:e0212815. PubMed Liu XC, et al. 2021. Front Aging Neurosci. 13:639318. PubMed Liu S, et al. 2022. J Neuroinflammation. 19:35. PubMed Shimada H, et al. 2022. Cell Rep Methods. 2:100289. PubMed Fassler M, et al. 2021. J Neuroinflammation. 18:19. PubMed Iacono D, et al. 2018. Parkinsonism Relat Disord. 58:63. PubMed Zhao P, et al. 2019. Front Behav Neurosci. 13:138. PubMed Saber M, et al. 2021. J Neurosci Res. 99:1136. PubMed Lu J, et al. 2021. EMBO Rep. 22:e52013. PubMed Kaneshiro N, et al. 2022. iScience. 25:103869. PubMed Sau-Yeen Loke, Peter Tsun-Hon Wong, Wei-Yi Ong 2017. Neurochem Int. 102:33-56. PubMed Shi H, et al. 2020. Acta Neuropathol Commun. 0.473611111. PubMed Prpar Mihevc S, et al. 2019. Front Neurosci. 13:604. PubMed Carrillo-Jiménez A, et al. 2019. Cell Rep. 29:697. PubMed Van Skike CE, et al. 2021. J Neurosci. 41:4305. PubMed Podracky CJ, et al. 2021. Nat Chem Biol. 17:317. PubMed Fixemer S, et al. 2022. Acta Neuropathol Commun. 10:36. PubMed Kim S, et al. 2020. Int J Mol Sci. 21:00. PubMed Jäkel L, et al. 2020. Mol Neurobiol. 57:2131. PubMed Meilandt WJ, et al. 2020. J Neurosci. 40:1956. PubMed Harper MM, et al. 2019. Invest Ophthalmol Vis Sci. 60:2716. PubMed Kim S, et al. 2019. Int J Mol Sci. 20. PubMed Zhu B, et al. 2022. Chem Sci. 13:8104. PubMed Prpar Mihevc S, et al. 2020. Front Vet Sci. 7:573155. PubMed Ezpeleta J, et al. 2019. Nat Commun. 10:3442. PubMed Pires G, et al. 2019. Acta Neuropathol Commun. 0.427083333. PubMed Wang ZH, et al. 2019. Cell Rep. 28:655. PubMed Lee KH, et al. 2017. Yonsei Med J. 58:1055. PubMed Libard S, et al. 2021. J Alzheimers Dis. 80:1003. PubMed Espinoza S, et al. 2022. Cells. 11:. PubMed Xu G, et al. 2020. Acta Neuropathol Commun. 8:43. PubMed Luo R, et al. 2020. Autophagy. 16:52. PubMed Song JX, et al. 2020. Aging Cell. 19:e13069. PubMed Li JG, et al. 2020. Mol Neurodegener. 15:01. PubMed Wang B, et al. 2017. Mol Psychiatry. 22:990. PubMed Carranza-Naval MJ, et al. 2022. Front Aging Neurosci. 13:741923. PubMed Jonson M, et al. 2018. Cell Chem Biol. 25:595. PubMed West E, et al. 2017. J Cell Sci. 130:3050. PubMed Rayaprolu S, et al. 2020. Mol Neurodegener. 15:28. PubMed Rivera I, et al. 2018. Cell Stress Chaperones. 23:269. PubMed Lo Cascio F, et al. 2020. J Biol Chem. 295:14807. PubMed Wang W et al. 2019. EBioMedicine. 42:174-187 . PubMed Catania M, et al. 2022. Mol Psychiatry. :. PubMed
RRID: AB_2734548 (BioLegend Cat. No. 800712) AB_2564633 (BioLegend Cat. No. 800701) AB_2564634 (BioLegend Cat. No. 800702) AB_662812 (BioLegend Cat. No. 800703)
Structure: Amyloid precursor protein is a 770 amino acid protein with a molecular mass of ~100 kD. According to the UniProtKB database, APP (ID# P05067) has 11 isoforms (34 to ~90 kD) and the 770 form has been designated as the canonical form. Isoform APP695 is the predominant form expressed in neuronal tissue. Isoforms APP751 and APP770 are widely expressed in non-neuronal cells. Isoform APP751 is the most abundant form in T-lymphocytes. Aβ denotes peptides of 36-43 amino acids generated from cleavage of APP by secretases. Aβ has an apparent molecular mass of about 4 kD.
Distribution: Tissue distribution: Primarily nervous system, but also adipose tissue, intestine, and muscle. Cellular distribution: Cytosol, endosomes, nucleus, plasma membrane, extracellular, and golgi apparatus.
Function: The normal function of Aβ is not well understood. Several potential physiological roles have been proposed, including: activation of kinase enzymes; protection against oxidative stress; regulation of cholesterol transport; transcription factor, and as an anti-microbial agent.
Biology Area: Cell Biology, Neurodegeneration, Neuroscience, Protein Misfolding and Aggregation
Molecular Family: APP/β-Amyloid
Antigen References: Kumar A, et al. 2015. Pharmacol Rep. 67(2):195. Sadigh-Eteghad S, et al. 2015. Med Princ Pract. 24(1):1 Hampel H, et al. 2015. Expert Rev Neruother. 15(1):83. Puig KL, et al. 2012. Exp Gerontol. 48(7): 608. Selkoe DJ, et al. 2016. EMBO Mol Med. 8(6):595. Walsh DM, et al. 2007. J Neurochem. 101(5):1172.
Gene ID: 351
UniProt: View information about beta-Amyloid 17-24 on UniProt.org
Clone: 4G8
Regulatory Status: RUO
Workshop: HCDM listed
Other Names: AAA, ABETA, ABPP, AD1, APPI, CTFgamma, CVAP, PN-II, PN2, Amyloid beta A4 protein, preA4, protease nexin-II, peptidase nexin-II, beta-amyloid peptide, alzheimer disease amyloid protein, cerebral vascular amyloid peptide, APP, Amyloid Precursor Protein
Previously: Signet Catalog# 9220-02Signet Catalog# 9220-05Signet Catalog# 9220-10Covance Catalog# SIG-39220
Isotype: Mouse IgG2b, κ