Biolegend, 800704, Biotin anti-β-Amyloid, 17-24 Antibody (Previously Covance catalog# SIG-39240), 200microl

Alzheimer’s disease is characterized by the accumulation of aggregated Aβ peptides in senile plaques and vascular deposits. Aβ peptides are derived from amyloid precursor proteins (APP) through sequential proteolytic cleavage of APP by β-secretases and γ-secretases generating diverse Aβ species. Aβ can aggregate to form soluble oligomeric species and insoluble fibrillar or amorphous assemblies. Some forms of the aggregated peptides are toxic to neurons.
200microl
Verified Reactivity: Human, Mouse
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: Phosphate-buffered solution (no preservatives or carrier proteins).
Preparation: The antibody was purified by affinity chromatography.
Concentration: 1 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check your vial or your CoA to find the most accurate storage condition for this antibody.
Application: IHC-P - Quality tested Direct ELISA - Verified
Recommended Usage: Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 1.0 - 2.0 µg/ml is suggested. For Direct ELISA applications, a concentration range of 0.5 - 2.0 µg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: This antibody is reactive to amino acid residues 17-24 of ß amyloid. The epitope lies within amino acids 18-22 of ß amyloid (VFFAE). 4G8 ß-amyloid antibody reacts to abnormally processed isoforms, as well as precursor forms. This antibody clone has been reported for use on IHC of free-floating sections in PBS containing 1% Triton incubated with 0.1 m citrate buffer (4). Additional reported applications (for the relevant formats) include: immunohistochemical staining on frozen tissue sections (IHC-F) and immunocytochemistry (ICC)
Additional Product Notes: View more applications data for this product in our Scientific Poster Library.
Application References(PubMed link indicates BioLegend citation): Poduslo JF, et al. 2004. Biochem. 43:6064. (IHC-F) PubMed Forny-Germano L, et al. 2014. J Neurosci. 34:13629. (IHC-Other) PubMed Vallino Costassa E, et al. 2016. J Alzheimers Dis. 51: 875-87. (IHC-P) PubMed Chen X, et al. 2013. Neurobiol Aging. 34:2370. (ICC) PubMed Hatami A, et al. 2016. J Alzheimers Dis. 50:517. (IHC-P) PubMed Kawarabayashi T, et al. 2001. J Neurosci 21:372 (IP) Fonte V, et al. 2002. PNAS. 110:4853 (IP)
Product Citations: Sun XY, et al. 2023. Signal Transduct Target Ther. 8:30. PubMed Lam S, et al. 2022. Acta Neuropathol Commun. 10:112. PubMed Townsend M, et al. 2010. J Pharmacol Exp Ther. 333:110:119. PubMed Jung ES, et al. 2022. Aging Cell. 21:e13623. PubMed Chen J, et al. 2019. Int J Mol Med. 44:313. PubMed Elmer BM, et al. 2019. PLoS One. 14:e0226245. PubMed Yao Q, et al. 2018. Acta Biochim Biophys Sin (Shanghai). 50(5):447-455. PubMed Watanabe H, et al. 2012. J Neurosci. 32:5085-5096. PubMed Hong J, et al. 2016. J Lipid Res. 57: 36 - 45. PubMed Clayton K, et al. 2021. Mol Neurodegener. 18:16. PubMed Bales K, et al. 2016. Brain. 139: 563 - 577. PubMed Luo M, et al. 2022. Genes Dis. 9:1315. PubMed Forgrave LM, et al. 2022. Clin Chem Lab Med. 60:198. PubMed Do TM, et al. 2020. Mol Ther Methods Clin Dev. 19:58. PubMed
RRID: AB_2564650 (BioLegend Cat. No. 800704) AB_2564651 (BioLegend Cat. No. 800705) AB_2564649 (BioLegend Cat. No. 800706)
Structure: Amyloid precursor protein is a 770 amino acid protein with a molecular mass of ~100 kD. According to the UniProtKB database, APP (ID# P05067) has 11 isoforms (34 to ~90 kD) and the 770 form has been designated as the canonical form. Isoform APP695 is the predominant form expressed in neuronal tissue. Isoforms APP751 and APP770 are widely expressed in non-neuronal cells. Isoform APP751 is the most abundant form in T-lymphocytes. Aβ denotes peptides of 36-43 amino acids generated from cleavage of APP by secretases. Aβ has an apparent molecular mass of about 4 kD.
Distribution: Tissue distribution: Primarily nervous system, but also adipose tissue, intestine, and muscle. Cellular distribution: Cytosol, endosomes, nucleus, plasma membrane, extracellular, and golgi apparatus.
Function: The normal function of Aβ is not well understood. Several potential physiological roles have been proposed, including: activation of kinase enzymes; protection against oxidative stress; regulation of cholesterol transport; transcription factor, and as an anti-microbial agent.
Biology Area: Cell Biology, Neurodegeneration, Neuroscience, Protein Misfolding and Aggregation
Molecular Family: APP/β-Amyloid
Antigen References: Kumar A, et al. 2015. Pharmacol Rep. 67(2):195. Sadigh-Eteghad S, et al. 2015. Med Princ Pract. 24(1):1 Hampel H, et al. 2015. Expert Rev Neruother. 15(1):83. Puig KL, et al. 2012. Exp Gerontol. 48(7): 608. Selkoe DJ, et al. 2016. EMBO Mol Med. 8(6):595. Walsh DM, et al. 2007. J Neurochem. 101(5):1172.
Gene ID: 351
UniProt: View information about beta-Amyloid 17-24 on UniProt.org
Clone: 4G8
Regulatory Status: RUO
Workshop: HCDM listed
Other Names: AAA, ABETA, ABPP, AD1, APPI, CTFgamma, CVAP, PN-II, PN2, Amyloid beta A4 protein, preA4, protease nexin-II, peptidase nexin-II, beta-amyloid peptide, alzheimer disease amyloid protein, cerebral vascular amyloid peptide, APP, Amyloid Precursor Protein
Previously: Signet Catalog# 9240-02Signet Catalog# 9240-05Signet Catalog# 9240-10Covance Catalog# SIG-39240
Isotype: Mouse IgG2b, κ
Q: How many biotin molecules are per antibody structure?
A: We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.