Biolegend, 800710, Anti-β-Amyloid, 17-24 Antibody (Previously Covance catalog# SIG-39200), 500microl

Alzheimer’s disease is characterized by the accumulation of aggregated Aβ peptides in senile plaques and vascular deposits. Aβ peptides are derived from amyloid precursor proteins (APP) through sequential proteolytic cleavage of APP by β-secretases and γ-secretases generating diverse Aβ species. Aβ can aggregate to form soluble oligomeric species and insoluble fibrillar or amorphous assemblies. Some forms of the aggregated peptides are toxic to neurons.
500microl
Verified Reactivity: Human, Mouse
Antibody Type: Monoclonal
Host Species: Mouse
Preparation: Ascites
Concentration: The concentration is not quantified as this product is sold as undiluted crude mouse ascites fluid. The concentration might vary from lot-to-lot and an estimated concentration would be 1-3 mg/ml.
Storage & Handling: Store at -20°C or below. Upon initial thawing, apportion into working aliquots and store at -20°C or below. Avoid repeated freeze-thaw cycles to prevent denaturing the antibody. Do not store in frost-free freezers.
Application: IHC-P - Quality tested Direct ELISA - Verified
Recommended Usage: Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a dilution range of 1:1000 - 1:5000 is suggested. For Direct ELISA applications, a dilution range of 1:100 - 1:1000 is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: This antibody is reactive to amino acid residues 17-24 of ß amyloid. The epitope lies within amino acids 18-22 of ß amyloid (VFFAE). 4G8 ß-amyloid antibody reacts to abnormally processed isoforms, as well as precursor forms. This antibody clone has been reported for use on IHC of free-floating sections in PBS containing 1% Triton incubated with 0.1 m citrate buffer (4).
Application References(PubMed link indicates BioLegend citation): Poduslo JF, et al. 2004. Biochem. 43:6064. (IHC-F) PubMed Forny-Germano L, et al. 2014. J Neurosci. 34:13629. (IHC-Other) PubMed Vallino Costassa E, et al. 2016. J Alzheimers Dis. 51: 875:87. (IHC-P) PubMed Chen X, et al. 2013. Neurobiol Aging. 34:2370. (ICC) PubMed Hatami A, et al. 2016. J Alzheimers Dis. 50:517. (IHC-P) PubMed
Product Citations: Bluhm A, et al. 2022. Biomolecules. 12:. PubMed Tournier BB, et al. 2023. Aging Brain. 2:100045. PubMed Sato W, et al. 2023. Commun Biol. 6:383. PubMed Klakotskaia D, et al. 2018. PLoS One. 8:8755. PubMed Choi S, et al. 2015. PLoS One. 10: 0143854. PubMed Woelfle S, et al. 2021. Front Cell Neurosci. 15:789903. PubMed Taddei RN, et al. 2022. Acta Neuropathol Commun. 10:72. PubMed Koronyo-Hamaoui M, et al. 2020. Brain. 143:336. PubMed Martin L, et al. 2021. Cell Reports. 35(6):109121. PubMed Gkanatsiou E, et al. 2021. J Neurochem. 159:258. PubMed Jiwaji Z, et al. 2022. Nat Commun. 13:135. PubMed Morales R, et al. 2021. Mol Psychiatry. 26:5690. PubMed Whitehouse I, et al. 2016. PLoS One. 11: 0159119. PubMed Chaudry O, et al. 2022. Sci Rep. 12:19129. PubMed Robertson AS, et al. 2017. Neuromuscul Disord. 27:635. PubMed Mu L, et al. 2022. Int J Mol Sci. 23:. PubMed Abramowski D, et al. 2012. J Neurosci. 32:1273-1283. PubMed Gamez N, et al. 2022. Cells. 11:. PubMed Schaefer P, et al. 2016. PLoS One. 11:e0168157. PubMed Lee CYD et al. 2018. Neuron. 97(5):1032-1048 . PubMed Lessard C, et al. 2015. PLoS One. 10: 0144758. PubMed Boeddrich A et al. 2018. Cell chemical biology. 26(1):109-120 . PubMed Zhang J, et al. 2021. iScience. 24:103327. PubMed Tang Z, et al. 2019. Nat Commun. 10:2173. PubMed Scholtzova H, et al. 2017. J Neurosci. 37(4):936-959. PubMed Maxwell AM, et al. 2021. Acta Neuropathol Commun. 9:201. PubMed
RRID: AB_2734547 (BioLegend Cat. No. 800708) AB_2565325 (BioLegend Cat. No. 800709) AB_2565326 (BioLegend Cat. No. 800710) AB_2565324 (BioLegend Cat. No. 800711)
Structure: Amyloid precursor protein is a 770 amino acid protein with a molecular mass of ~100 kD. According to the UniProtKB database, APP (ID# P05067) has 11 isoforms (34 to ~90 kD) and the 770 form has been designated as the canonical form. Isoform APP695 is the predominant form expressed in neuronal tissue. Isoforms APP751 and APP770 are widely expressed in non-neuronal cells. Isoform APP751 is the most abundant form in T-lymphocytes. Aβ denotes peptides of 36-43 amino acids generated from cleavage of APP by secretases. Aβ has an apparent molecular mass of about 4 kD.
Distribution: Tissue distribution: Primarily nervous system, but also adipose tissue, intestine, and muscle. Cellular distribution: Cytosol, endosomes, nucleus, plasma membrane, extracellular, and golgi apparatus.
Function: The normal function of Aβ is not well understood. Several potential physiological roles have been proposed, including: activation of kinase enzymes; protection against oxidative stress; regulation of cholesterol transport; transcription factor, and as an anti-microbial agent.
Biology Area: Cell Biology, Neurodegeneration, Neuroscience, Protein Misfolding and Aggregation
Molecular Family: APP/β-Amyloid
Antigen References: Kumar A, et al. 2015. Pharmacol Rep. 67(2):195. Sadigh-Eteghad S, et al. 2015. Med Princ Pract. 24(1):1 Hampel H, et al. 2015. Expert Rev Neruother. 15(1):83. Puig KL, et al. 2012. Exp Gerontol. 48(7): 608. Selkoe DJ, et al. 2016. EMBO Mol Med. 8(6):595. Walsh DM, et al. 2007. J Neurochem. 101(5):1172.
Gene ID: 351
UniProt: View information about beta-Amyloid 17-24 on UniProt.org
Clone: 4G8
Regulatory Status: RUO
Workshop: HCDM listed
Other Names: AAA, ABETA, ABPP, AD1, APPI, CTFgamma, CVAP, PN-II, PN2, Amyloid beta A4 protein, preA4, protease nexin-II, peptidase nexin-II, beta-amyloid peptide, alzheimer disease amyloid protein, cerebral vascular amyloid peptide, APP, Amyloid Precursor Protein
Previously: Signet Catalog# 9200-02Signet Catalog# 9200-05Signet Catalog# 9200-10Covance Catalog# SIG-39200
Isotype: Mouse IgG2b, κ