Biolegend, 803310, Alexa Fluor® 647 anti-ApoE Antibody, 100μg

Apo-E is a class of apolipoprotein primarily synthesized in the liver, but is also found in other tissues such as brain, kidneys, and spleen. Apo-E is a protein component of serum chylomicron and lipoprotein particles, playing essential roles in cholesterol transportation, homeostasis, and metabolism. The expression of Apo-E is induced by cholesterol-rich diets and hyperlipoproteinemia. In the central nervous system, Apo-E is produced by astrocytes and microglia. The Apo-E-containing lipoprotein particles play important functions in brain injury repair, neurogenesis, and synaptic plasticity. Apo-E has been found to be involved in the regulation of amyloid-β aggregation and clearance. Genetic polymorphisms of Apo-E are associated with neuron degenerative disorders including Alzheimer's disease.
100μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: This antibody was raised against a peptide sequence corresponding to aa 141-160 of human Apo-E.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: IHC-P - Quality tested ICC - Verified
Recommended Usage: Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 5.0 - 10.0 µg/mL is suggested. For immunocytochemistry, a concentration range of 5.0 - 10.0 μg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses
Excitation Laser: Red Laser (633 nm)
Additional Product Notes: For use in immunohistochemistry on formalin-fixed paraffin-embedded tissue (IHC-P), it is recommended to perform antigen retrieval using either Citrate Buffer, 10X (Cat. No. 420902) or Tris-EDTA pH 9.0 Antigen Retrieval Buffer (10X) (Cat. No. 422704). For use in immunocytochemistry (ICC), it is recommended to fix/permeabilize with any of the following: -100% ice-cold methanol only -Fixation buffer (Cat. No. 420801) with 100% ice-cold methanol Fixation/permeabilization using Fixation buffer (Cat. No. 420801) with 0.5 % Triton X-100 is not recommended.
Application References(PubMed link indicates BioLegend citation): Munoz DG and Feldman H. 2000. CMAJ. 162(1):65-72. Hagberg JM, et al. 2000. Physiol Genomics. 4(2):101-108. Saunders AM. 2000. J Neuropathol Exp Neurol. 59(9):751-8. Golabek AA, et al. 2000. Biophys J. 79(2):1008-15. Zunarelli E, et al. 2000. Breast Cancer Res Treat. 63(3):193-8. Zunarelli E, et al. 2000. Clin Neuropathol. 19(1):1-6. Tomiyama T, et al. 1999. Cell Mol Life Sci. 6(3-4):268-79. Golabek AA., et al. 1996. J Biol Chem. 271(18):10602-6.
RRID: AB_3720618 (BioLegend Cat. No. 803309) AB_3720618 (BioLegend Cat. No. 803310)
Biology Area: Cell Biology, Neurodegeneration, Neuroscience, Protein Trafficking and Clearance
Gene ID: 348
UniProt: View information about Apo E on UniProt.org
Clone: D6E10
Regulatory Status: RUO
Other Names: Apolipoprotein E, apolipoprotein E3, AD2, LDLCQ5, LPG, ApoE
Isotype: Mouse IgG1, κ