Biolegend, 803402, Purified anti-Apo E Antibody (Previously Covance catalog# SIG-39750), 500microl

Apo-E is a class of apolipoprotein primarily synthesized in the liver, but is also found in other tissues such as brain, kidneys, and spleen. Apo-E is a protein component of serum chylomicron and lipoprotein particles, playing essential roles in cholesterol transportation, homeostasis, and metabolism. The expression of Apo-E is induced by cholesterol-rich diets and hyperlipoproteinemia. In the central nervous system, Apo-E is produced by astrocytes and microglia. The Apo-E-containing lipoprotein particles play important functions in brain injury repair, neurogenesis, and synaptic plasticity. Apo-E has been found to be involved in the regulation of amyloid-β aggregation and clearance. Genetic polymorphisms of Apo-E are associated with neuron degenerative disorders including Alzheimer's disease.
500microl
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: This antibody was raised against a synthetic peptide corresponding to amino acids surrounding the polymorphic amino acid position 158 of Apo-E.
Formulation: Phosphate-buffered solution (no preservatives or carrier proteins).
Preparation: The antibody was purified by affinity chromatography.
Concentration: 1 mg/ml
Storage & Handling: This antibody should be handled aseptically as it is free of preservatives such as Sodium Azide. Store this antibody undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check the vial label or CoA to find the proper storage conditions.
Application: WB - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1.0 - 5.0 µg/ml. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: This antibody is effective in immunoblotting (WB).Clone E6D7 recognizes the E2, E3, and E4 isoforms of apolipoprotein E.
Application References(PubMed link indicates BioLegend citation): Munoz DG, Feldman H. 2000. CMAJ. 162(1):65-72. Hagberg JM, et al. 2000. Physiol Genomics 4(2):101-108. Saunders AM. 2000. J. Neuropathol. Exp. Neurol. 59(9):751-8. Golabek AA, et al. 2000. Biophys. J. 79(2):1008-15. Zunarelli E, et al. 2000. Breast Cancer Res. Treat. 63(3):193-8. Zunarelli E, et al. 2000. Clin. Neuropathol. 19(1):1-6. Tomiyama T, et al. 1999. Cell Mol. Life Sci. 56(3-4):268-79. Golabek AA., et al. 1999. J. Biol. Chem. 271(18):10602-6.
RRID: AB_2564664 (BioLegend Cat. No. 803401) AB_2564663 (BioLegend Cat. No. 803403)
Biology Area: Cell Biology, Neurodegeneration, Neuroscience, Protein Trafficking and Clearance
Molecular Family: Apolipoproteins
Gene ID: 348
UniProt: View information about Apo E on UniProt.org
Clone: E6D7
Regulatory Status: RUO
Other Names: Apolipoprotein E, apolipoprotein E3, AD2, LDLCQ5, LPG, ApoE
Previously: Signet Catalog# 9750-02Signet Catalog# 9750-05Signet Catalog# 9750-10Covance Catalog# SIG-39750
Isotype: Mouse IgG1, κ