Biolegend, 805407, HRP anti-β-Amyloid, 1-40 Antibody (Previously Covance catalog# SIG-39146), 200microl

Amyloid beta (Aβ or Amyloid beta) denotes peptides of 36-43 amino acids in length that are crucially involved in Alzheimer's disease as the main component of the amyloid plaques found in the brains of Alzheimer patients. The peptides result from the amyloid precursor protein (APP), which is cut by certain enzymes to yield Aβ. Aβ molecules can aggregate to form oligomers (known as "seeds") which are believed to be able to induce other Aβ molecules to also take the misfolded oligomeric form, leading to a chain reaction akin to a prion infection. The seeds or the resulting amyloid plaques are toxic to nerve cells. The other protein implicated in Alzheimer's disease, tau protein, also forms such prion-like misfolded oligomers, and there is some evidence that misfolded Aβ can induce tau to misfold.
200microl
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: This antibody is provided in 50% glycerol in aqueous buffered solutions with preservatives.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: Upon receipt, the antibody solution should be stored undiluted at -20°C, and protected from prolonged exposure to light. For storage of this antibody when diluted below a concentration of 50 µg/mL, a carrier protein such as 2% bovine serum albumin is required.
Application: IHC-P - Quality tested Dot Blot, ELISA, IP - Reported in the literature, not verified in house
Recommended Usage: Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 1.0 - 10 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: Clone 11A50-B10 is reactive to the C-terminus of beta amyloid and is specific for the isoform ending at the 40th amino acid.
Application References(PubMed link indicates BioLegend citation): Watts JC, et al. 2014. Proc. Natl. Sci. USA 28:10323. (IHC-P) PubMed Diomede L, et al. 2014. Neurobiol. Dis. 62:521. (Dot Blot) PubMed Watanabe H, et al. 2012. J Neurosci. 15:5085. (ELISA) PubMed Portelius E, et al. 2006. J. Proteome Res. 4:1010. (IP) Vingtdeux V, et al. 2011. FASEB J. 25:219. (ELISA) PubMed Ohmi K, et al. 2011. PLoS One 11:e27461. (IHC-Other) PubMed
Product Citations: Kotiya D, et al. 2023. J Biol Chem. 104682:299. PubMed Casali BT, et al. 2020. Neurobiol Dis. 142:104956. PubMed Casali BT, et al. 2018. J Neuroinflammation. 15:43. PubMed Cheng-Hathaway PJ, et al. 2018. Mol Neurodegener. 13:e0200060. PubMed
RRID: AB_2564979 (BioLegend Cat. No. 805407)
Biology Area: Cell Biology, Neurodegeneration, Neuroscience, Protein Misfolding and Aggregation
Molecular Family: APP/β-Amyloid
Gene ID: 351
UniProt: View information about beta-Amyloid 1-40 on UniProt.org
Clone: 11A50-B10
Regulatory Status: RUO
Other Names: AAA, ABETA, ABPP, AD1, APPI, CTFgamma, CVAP, PN-II, PN2, Amyloid beta A4 protein, preA4, protease nexin-II, peptidase nexin-II, beta-amyloid peptide, alzheimer disease amyloid protein, cerebral vascular amyloid peptide, APP, Amyloid Precursor Protein
Previously: Signet Catalog# 9146-02Covance Catalog# SIG-39146
Isotype: Mouse IgG1, κ