Biolegend, 805503, Purified anti-β-Amyloid, 1-42 Antibody (Previously Covance catalog# SIG-39142), 1ml

Amyloid beta (Aβ or Abeta) denotes peptides of 36–43 amino acids in length that are crucially involved in Alzheimer's disease as the main component of the amyloid plaques found in the brains of Alzheimer patients. The peptides result from the amyloid precursor protein (APP), which is cut by certain enzymes to yield Aβ. Aβ molecules can aggregate to form oligomers (known as "seeds") which are believed to be able to induce other Aβ molecules to also take the misfolded oligomeric form, leading to a chain reaction akin to a prion infection. The seeds or the resulting amyloid plaques are toxic to nerve cells. The other protein implicated in Alzheimer's disease, tau protein, also forms such prion-like misfolded oligomers, and there is some evidence that misfolded Aβ can induce tau to misfold.
1ml
Verified Reactivity: Human, Mouse, Rat
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: Phosphate-buffered solution (no preservatives or carrier proteins).
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: This antibody should be handled aseptically as it is free of preservatives such as Sodium Azide. Store this antibody undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check the vial label or CoA to find the proper storage conditions.
Application: IHC-P - Qualty tested Direct ELISA, WB - Verified IHC-F, ELISA - Reported in the literature, not verified in house
Recommended Usage: Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 0.2 - 5.0 µg/ml is suggested. For Direct ELISA, a concentration range of 0.015 - 1.5 µg/ml is suggested. For Western blotting, the suggested use of this reagent is 1.0 - 5.0 µg/ml. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: This antibody is reactive to the C-terminus of beta amyloid and is specific for the isoform ending at the 42nd amino acid.
Application References(PubMed link indicates BioLegend citation): Helwig M, et al. 2013. J. Biol. Chem. 288:1114. (IHC-P) Takami M, et al.2009. J. Neurosci.; 29: 13042. (IHC-P) Cenini G, et al. 2012. Biochim Biophys Acta. 1822(2):130. (ELISA) PubMed Sundaram RK, et al. 2012. Int. J. Pept. Res. Ther. 18(2):99. (ELISA) PubMed Savage MJ, et al. 2014. J. Neurosci. 8:2884. (ELISA) PubMed Mastrangelo MA, Bowers WJ. 2008. BMC Neurosci. 9:81. (IHC-F) PubMed
Product Citations: Barbato C, et al. 2020. Molecular Neurobiology. 57(7):3183-3194. PubMed Bishay J, et al. 2022. Sci Rep. 12:15287. PubMed Jin J, et al. 2023. Gut Microbes. 15:2167172. PubMed Willumsen N, et al. 2023. Brain Commun. 5:fcac321. PubMed Jordà-Siquier T, et al. 2022. Alzheimers Dement. 18:2099. PubMed Nick H, et al. 2022. Front Aging Neurosci. 14:1025402. PubMed Vingtdeux V, et al. 2011. FASEB J. 25:219-231. PubMed Kim C, et al. 2017. Mol Neurobiol. 10.1007/s12035-017-0683-3. PubMed Townsend M, et al. 2010. J Pharmacol Exp Ther. 333:110:119. PubMed Ruiz-Riquelme A, et al. 2021. Acta Neuropathol Commun. 9:83. PubMed Helwig M, et al. 2013. J Biol Chem. 288:1114-1124. PubMed Moreno F, et al. 2017. PLoS One. 12(6):e0178093. PubMed Koh HS, et al. 2021. Int J Mol Sci. 22:. PubMed Gutknecht MF, et al. 2022. Cell Rep Methods. 2:100214. PubMed Owen JE, et al. 2021. Sleep. 44:zsab057. PubMed Kim H, et al. 2021. Front Aging Neurosci. 13:709091. PubMed Olah M, et al. 2020. Nat Commun. 4.714583333. PubMed Augé E, et al. 2017. Sci Rep. 7:41807. PubMed Shi H, et al. 2020. Acta Neuropathol. 139:813. PubMed Takami M, et al. 2009. J Neurosci. 29:13042-13052. PubMed Maheshwari M, et al. 2010. Biochemistry. 49:10371-10380. PubMed Sudol K, et al. 2009. Mol Ther. 17:2031-2040. PubMed Lee CYD et al. 2018. Neuron. 97(5):1032-1048 . PubMed Plowey ED, et al. 2022. Acta Neuropathol. 144:143. PubMed Kim K, et al. 2020. Nat Commun. 0.540972222. PubMed Chan HN, et al. 2019. Theranostics. 9:2939. PubMed Kommaddi RP et al. 2018. The Journal of Neuroscience. 38(5):1085-1099 . PubMed Piccirillo S, et al. 2022. Cell Death Discov. 8:391. PubMed Li K, et al. 2020. ACS Chemical Neuroscience. 11(24):4316-4328. PubMed Ohmi K, et al. 2011. PLoS One. 6:e27461. PubMed Vassar W 2013. Mol Neurodegener. 8:2. PubMed Faraz A, et al. 2017. Antioxid Redox Signal. . 10.1089/ars.2016.6860. PubMed Maxwell AM, et al. 2021. Acta Neuropathol Commun. 9:201. PubMed Parker H, et al. 2020. EMBO Mol Med. 12:e11185. PubMed Ruiz–Riquelme A, et al. 2018. Acta Neuropathol Commun. 6:26. PubMed
RRID: AB_2783381 (BioLegend Cat. No. 805509) AB_2564683 (BioLegend Cat. No. 805501) AB_2564684 (BioLegend Cat. No. 805502) AB_2564682 (BioLegend Cat. No. 805503)
Structure: Amyloid precursor protein is a 770 amino acid protein with a molecular mass of ~100 kD. According to the UniProtKB database, APP (ID# P05067) has 11 isoforms (34 to ~90 kD) and the 770 form has been designated as the canonical form. Isoform APP695 is the predominant form expressed in neuronal tissue. Isoforms APP751 and APP770 are widely expressed in non-neuronal cells. Isoform APP751 is the most abundant form in T-lymphocytes. Aβ denotes peptides of 36-43 amino acids generated from cleavage of APP by secretases. Aβ has an apparent molecular mass of about 4 kD.
Distribution: Tissue sources: Primarily nervous system, but also adipose tissue, intestine, muscle. Distribution: cytosol, endosomes, nucleus, plasma membrane, extracellular, and golgi apparatus.
Function: The normal function of Aβ is not well understood. Several potential physiological roles have been proposed, including: activation of kinase enzymes; protection against oxidative stress; regulation of cholesterol transport; transcription factor, and as an anti-microbial agent.
Biology Area: Cell Biology, Neurodegeneration, Neuroscience, Protein Misfolding and Aggregation
Molecular Family: APP/β-Amyloid
Antigen References: Kumar A, et al. 2015. Pharmacol. Rep. 67(2):195. Sadigh-Eteghad S, et al. 2015. Med. Princ. Pract. 24(1):1 Hampel H, et al. 2015. Expert Rev. Neurother. 15(1):83. Puig KL, et al. 2012. Exp. Gerontol. 48(7): 608. Selkoe DJ, et al. 2016. EMBO Mol. Med. 8(6):595. Walsh DM, et al. 2007. J. Neurochem. 101(5):1172.
Gene ID: 351
UniProt: View information about beta-Amyloid 1-42 on UniProt.org
Clone: 12F4
Regulatory Status: RUO
Other Names: AAA, ABETA, ABPP, AD1, APPI, CTFgamma, CVAP, PN-II, PN2, Amyloid beta A4 protein, preA4, protease nexin-II, peptidase nexin-II, beta-amyloid peptide, alzheimer disease amyloid protein, cerebral vascular amyloid peptide, APP, Amyloid Precursor Protein
Previously: Signet Catalog# 9142-02Signet Catalog# 9142-05Signet Catalog# 9142-10Covance Catalog# SIG-39142
Isotype: Mouse IgG1, κ