Biolegend, 807804, Purified anti-α-Synuclein, 103-108 Antibody (Previously Covance catalog# SIG-39730), 25microl

α-Synuclein is expressed principally in the nervous system, but it is also produced in other tissues, including  the skin. In the brain, the protein is primarily neuronal, but it is also present in glia. Neuronal α-synuclein is concentrated in presynaptic nerve terminals, interacts with plasma membrane phospholipids, and is also present in nuclei and mitochondria. At least three isoforms of synuclein are produced through alternative splicing. The most common isoform is a 140 amino acid-long transcript. Other isoforms are a-synuclein-126, lacking residues 41-54; and α-synuclein-112, which lacks residues 103-130. α-Synuclein’s physiological role is poorly understood, but the protein has been implicated in regulating dopamine release and transport, synaptic vesicle clustering, and functioning as a SNARE-complex chaperone. α-Synuclein fibrils are major component of the intracellular Lewy bodies that are associated with Parkinson's disease, Lewy body dementia, and multiple system atrophy.
25microl
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: This antibody was developed using a purified, E. coli produced human α-synuclein.
Formulation: Phosphate-buffered solution (no preservatives or carrier proteins).
Preparation: The antibody was purified by affinity chromatography.
Concentration: 1 mg/mL
Storage & Handling: This antibody should be handled aseptically as it is free of preservatives such as Sodium Azide. Store this antibody undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check the vial label or CoA to find the proper storage conditions.
Application: IHC-P - Quality tested WB - Verified ELISA - Reported in the literature, not verified in house
Recommended Usage: Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 1.0 - 2.5 µg/mL is suggested. For Western blotting, the suggested use of this reagent is 1.0 - 10.0 µg per mL. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: This antibody reacts with human a-synuclein. It does not react with mouse or rat a-synuclein. The epitope lies within amino acids 103-108 of a-synuclein. Additional reported applications (for the relevant formats) include: ELISA2, Western blotting1,2,3,4,6,8, and immunoprecipitation4.
Application References(PubMed link indicates BioLegend citation): Lahut S, et al. 2017. Dis Model Mech. (WB) PubMed Landeck N, et al. 2016. Mol Neurodegener. 11(1): 61. (ELISA, WB) PubMed Moussaud S, et al. 2015. Expert Opin Ther Targets. 19(5): 589. (WB) PubMed Helferich AM., et al. 2015. Mol Neurodegener. 10(1): 66. (IHC, IP, WB) PubMed Bodis‐Wollner I, et al. 2014. Ann Neurol. 75(6): 964. (IHC) Shen W, et al. 2014. J Neurochem. 129(5): 884. (WB) Fagerqvist T, et al. 2013. J Neurochem. 126(1): 131. (IHC) Ebrahimi-Fakhari D, et al. 2011. J Neurosci. 31(41): 14508. (WB) PubMed Welander H, et al. 2011. Biochem Biophys Res Commun. 412(1): 32. (IHC)
Product Citations: Schaser AJ, et al. 2019. Sci Rep. 9:10919. PubMed Kiechle M, et al. 2019. Cell Reports. 29(9):2862-2874.e9. PubMed Cariulo C, et al. 2019. Front Neurosci. 13:889. PubMed Butkovich LM, et al. 2020. J Neurosci. 7559:40. PubMed Zhao J, et al. 2021. Acta Neuropathol. 142:807. PubMed Heras–Garvín A, et al. 2019. Mov Disord. 34:255. PubMed Tanudjojo B, et al. 2021. Nat Commun. 12:3817. PubMed Phan JA, et al. 2017. Sci Rep. 7:6363. PubMed Scheiblich H, et al. 2021. Cell. 184:5089. PubMed Van der Perren A, et al. 2020. Acta Neuropathol. 139:977. PubMed Jiang P, et al. 2017. Sci Rep. 7:7690. PubMed Zhao N, et al. 2020. Sci Transl Med. 12:00. PubMed
RRID: AB_2734561 (BioLegend Cat. No. 807804) AB_2564730 (BioLegend Cat. No. 807801) AB_2564731 (BioLegend Cat. No. 807802) AB_2564729 (BioLegend Cat. No. 807803)
Structure: α-Synuclein’s canonical isoform is a 140 amino acid protein with an apparent molecular mass of 14 kD.
Distribution: Tissue distribution: Primarily nervous system, but lower expression in other tissues. Cellular distribution: Cytoskeleton, cytosol, lysosome, mitochondria, nucleus, plasma membrane, and extracellular.
Biology Area: Cell Biology, Neurodegeneration, Neuroscience, Protein Misfolding and Aggregation
Molecular Family: α-Synuclein
Antigen References: Lee JM, et al. 2017. J Neuropathol Exp Neurol. 76(1):2. Kang SS, et al. 2017. Proc Natl Acad Sci. 114(5): 1183. Valdes P, et al. 2016. Front Neuroanat. 10:123. Emamzadeh FN. 2016. J Res Med Sci. 9(21):29.
Gene ID: 6622
UniProt: View information about alpha-Synuclein 103-108 on UniProt.org
Clone: 4B12/Synuclein
Regulatory Status: RUO
Other Names: NACP, PARK1, PARK4, PD1, Synuclein alpha-140, non-A4 component of amyloid, alpha-synuclein, isoform NACP140, non-A beta component of AD amyloid Parkinson disease
Previously: Signet Catalog# 9730-02Signet Catalog# 9730-05Signet Catalog# 9730-10Covance Catalog# SIG-39730
Isotype: Mouse IgG1, κ