Biolegend, 810101, Purified anti-S100A4 Antibody (Previously Covance catalog# PRB-497P), 250microl

The S100A4 protein belongs to the S100 subfamily, which has grown to be one of the largest subfamilies of the EF-hand Ca2+-binding proteins. The S100 proteins are small (10-12 kD), acidic proteins sharing 25-65% sequence homology. A majority of the S100 genes cluster on human chromosome 1q21. The discovery of this cluster has provided the basis for a new nomenclature for S100 proteins encoded by genes in the 1q21 region. These proteins are now designated S100A1-S100A13. In immunoblotting analysis using recombinant S100 proteins (S100A1, S100A2, S100A4, S100A6 and S100B) only the corresponding antigen (S100A4) was recognized. No cross-reaction with the other S100 proteins was observed.
250microl
Verified Reactivity: Human, Mouse, Rat
Reported Reactivity: Cat, Cow, Dog
Antibody Type: Polyclonal
Host Species: Rabbit
Immunogen: This antibody was raised against purified recombinant human S100A4 monomer expressed by E. coli.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.1 M NaCl, 0.015 M sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 1.0 mg/ml
Storage & Handling: Store between 2 - 8°C
Application: IHC-P, ICC
Recommended Usage: Each lot of this antibody is quality control tested by immunohistochemical staining. The optimal working dilution should be determined for each specific assay condition. • IHC-P: 1:100 - 1:200 for Biotin based detection systems such as USA Ultra Streptavidin Detection (929501). Tissue Sections: Formalin-fixed, paraffin-embedded tissues Pretreatment: For optimal staining, the sections should be pretreated with an antigen unmasking solution such as Citrate Buffer Retrieval Solution (928502). Incubation: 20 minutes at room temperature.
Application Notes: This antibody is effective in immunocytochemistry (ICC) and immunohistochemistry (IHC). Predicted Molecular Weight = 10 - 12 kD Postive Tissue: Tonsil (human) This product may contain other non-IgG subtypes.
Application References(PubMed link indicates BioLegend citation): Schäfer BW, Heizmann CW. The S100 family of EF-hand calcium-binding proteins: functions and pathology. Trends Biochem Sci 21:134-40, 1996. Engelkamp D, Schäfer BW, Mattei MG, Erne P, Heizmann CV. Six S100 genes are clustered on human chromosome 1q21: identification of two genes coding for the two previously unreported calcium-binding proteins S100D and S100E. Proc Natl Acad Sci 90:6547-51, 1993.
Product Citations: Seldin L, et al. 2020. Developmental Cell. 55(5):558-573.e6. PubMed Lee DF, et al. 2018. Sci Rep. 8:11927. PubMed Chen L, et al. 2020. Cancer Immunol Res. 1381:8. PubMed
RRID: AB_2564748 (BioLegend Cat. No. 810101)
Biology Area: Cell Biology, Neuroinflammation, Neuroscience
Molecular Family: Immune Checkpoint Receptors
Gene ID: 20198
UniProt: View information about S100A4 on UniProt.org
Clone: S100A4
Regulatory Status: RUO
Other Names: Protein S100-A4, calvasculin, protein 18A2, protein Mts1, metastatic cell protein, S100 calcium-binding protein A4, placental calcium-binding protein, leukemia multidrug resistance associated protein, 18A2, 42A, CAPL, FSP1, MTS1, P9KA, PEL98
Previously: Covance Catalog# PRB-497P
Isotype: Rabbit Polyclonal IgG