Biolegend, 812101, Purified anti-β-Amyloid, x-42 Antibody (Previously Covance catalog# SIG-39169), 50μg

Alzheimer’s disease is characterized by the accumulation of aggregated Aβ peptides in senile plaques and vascular deposits. Aβ peptides are derived from amyloid precursor protein (APP) through sequential proteolytic cleavage of APP by β- and γ-secretases generating diverse Aβ species. Aβ can aggregate to form soluble oligomeric species and insoluble fibrillar or amorphous assemblies. Some forms of the aggregated peptides are toxic to neurons.
50μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Rabbit
Formulation: Phosphate-buffered solution (no preservatives or carrier proteins).
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: This antibody should be handled aseptically as it is free of preservatives such as Sodium Azide. Store this antibody undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check the vial label or CoA to find the proper storage conditions.
Application: IHC-P - Quality tested WB - Reported in the literature, not verified in house
Recommended Usage: Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 5.0 - 10 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: This antibody is effective in immunohistochemistry (IHC-P) and immunoblotting (WB). This antibody is reactive to the C-terminus of beta amyloid and specific for the isoform that ends at the 42nd amino acid.
Application References(PubMed link indicates BioLegend citation): Currais A, et al. 2012. Aging Cell 6:1017. (IHC) PubMed Mastrangelo MA, Bowers WJ. 2008. BMC Neurosci. 9:81. (IHC) PubMed
Product Citations: Currais A, et al. 2012. Aging Cell. 11:1017-1026. PubMed Samuel I, et al. 2022. Front Neurosci. 937663:16. PubMed Bowers M 2008. BMC Neurosci. 9:81. PubMed
RRID: AB_2564763 (BioLegend Cat. No. 812101)
Disclaimer: Covered by US patents 5,675,063 and 7,429,487. Sold under license from Epitomics.
Structure: Expected MW: 4 kD
Distribution: Tissue distribution: Primarily nervous system, but also adipose tissue, intestine, muscle. Cellular distribution: Cytosol, endosomes, nucleus, plasma membrane, extracellular, and golgi apparatus.
Function: The normal function of Aβ is not well understood. Several potential physiological roles have been proposed, including: activation of kinase enzymes; protection against oxidative stress; regulation of cholesterol transport; transcription factor, and as an anti-microbial agent.
Biology Area: Cell Biology, Neurodegeneration, Neuroscience, Protein Misfolding and Aggregation
Molecular Family: APP/β-Amyloid
Gene ID: 351
UniProt: View information about beta-Amyloid x-42 on UniProt.org
Clone: 1-11-3
Regulatory Status: RUO
Other Names: Amyloid beta A4 protein, preA4, protease nexin-II, peptidase nexin-II, beta-amyloid peptide, alzheimer disease amyloid protein, cerebral vascular amyloid peptide, APP, Amyloid Precursor Protein
Previously: Covance Catalog# SIG-39169
Isotype: Rabbit IgG