Biolegend, 813701, Purified anti-Apo E4 Antibody (Previously Covance catalog# SIG-39754), 200microl

Apolipoprotein E (ApoE) belongs to a class of apolipoproteins that binds to lipids to form lipoproteins. ApoE is produced by the liver and macrophages in peripheral tissues where it regulates cholesterol metabolism. In the central nervous system, ApoE is primarily produced by astrocytes and plays an important role in transporting cholesterol to neurons via ApoE receptors. ApoE has three major isoforms corresponding to three alleles (ApoE2, ApoE3 and ApoE4) that differ from each other by only one or two amino acids at positions 112 and 158. ApoE4 is one of the largest known genetic risk factors for late-onset Alzheimer's disease. Further, ApoE4 has been implicated in several other diseases such as atherosclerosis, impaired cognitive function and multiple sclerosis.
200microl
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: Phosphate-buffered solution (no preservatives or carrier proteins).
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: This antibody should be handled aseptically as it is free of preservatives such as Sodium Azide. Store this antibody undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check the vial label or CoA to find the proper storage conditions.
Application: ELISA Capture - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by ELISA assay. For ELISA Capture applications, a concentration range of 3 - 5 µg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: This antibody is specific for the E4 isoform of apolipoprotein E.
Product Citations: Jablonski AM, et al. 2021. Sci Rep. 11:3438. PubMed
RRID: AB_2564775 (BioLegend Cat. No. 813701) AB_2564774 (BioLegend Cat. No. 813702)
Structure: Predicted MW = 34 kD
Biology Area: Cell Biology, Neurodegeneration, Neuroscience, Protein Trafficking and Clearance
Gene ID: 348
UniProt: View information about Apo E4 on UniProt.org
Clone: 9D11
Regulatory Status: RUO
Other Names: Apolipoprotein E, apoloprotein E4
Previously: Covance Catalog# SIG-39754
Isotype: Mouse IgG1, κ