Biolegend, 814306, HRP anti-Tau, 189-195 Antibody, 100μg

Tau proteins are microtubule-associated protein (MAPs) which are abundant in neurons of the central nervous system, but are also expressed at very low levels in CNS astrocytes and oligodendrocytes and elsewhere. One of tau's main functions is to modulate the stability of axonal microtubules. Tau is active primarily in the distal portions of axons providing microtubule stabilization as well as flexibility. Pathologies and dementias of the nervous system such as Alzheimer's disease feature tau proteins that have become defective and no longer stabilize microtubules properly. As a result, tau forms aggregates with specific structural properties referred to as Paired Helical Filaments (PHFs) that are a characteristic of many different types of dementias, known as tauopathies.
100μg
Verified Reactivity: Human, Mouse, Rat
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: This monoclonal antibody was raised against full-length human tau.
Formulation: This antibody is provided in 50% glycerol in aqueous buffered solutions with preservatives.
Preparation: The antibody was purified by affinity chromatography and conjugated with HRP under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: Upon receipt, the antibody solution should be stored undiluted at -20°C, and protected from prolonged exposure to light.
Application: WB - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.2 - 1.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: This antibody is effective in immunoblotting (WB). BioLegend is the Exclusive Provider of this Antibody Product
Application References(PubMed link indicates BioLegend citation): Michalski V, et.al. Neurobiol Aging. 2015. 36(12):3131. (WB) PubMed
Product Citations: Serafín V, et al. 2020. Anal Bioanal Chem. 413:799. PubMed
RRID: AB_2734569 (BioLegend Cat. No. 814306)
Structure: Unmodified Tau isoforms have an apparent molecular weight ranging from 33-79 kD. Additional high and low molecular weight Tau species have been observed in brain tissues.
Distribution: Tissue distribution: Central nervous system, peripheral ganglia and nerves, kidney, skeletal, and heart muscle. Cellular distribution: Cytoskeleton, nucleus, plasma membrane, and cytosol.
Function: Tau promotes microtubule assembly and stability. The short tau isoforms allow plasticity of the cytoskeleton whereas the longer isoforms may preferentially play a role in its stabilization.
Interaction: Tau interacts with: Sequestosome-1, Peptidyl-prolyl cis-trans isomerase FKBP4, Casein kinase I isoform delta, Serine/threonine-protein kinase Sgk1, Laforin, and alpha-synuclein.
Biology Area: Cell Biology, Neurodegeneration, Neuroscience, Protein Misfolding and Aggregation
Molecular Family: Tau
Gene ID: 4137
UniProt: View information about Tau 189-195 on UniProt.org
Clone: 39E10
Regulatory Status: RUO
Other Names: Microtubule-associated protein tau, PHF-tau, Paired Helical Filament tau
Isotype: Mouse IgG1, κ