Biolegend, 815701, Purified anti-Histone H3 Nonmethyl (Lys9) Antibody (Previously Covance catalog# MMS-5083), 100microl

Histone H3 is a 17 kD nuclear protein that is a component of an octamer containing pairs of each of four core histones (H2A, H2B, H3, H4). The core histones create nucleosome structure of chromosomal fiber in eukaryotes and are dynamic in gene regulation. The histones exhibit cell cycle-dependent transcriptional regulation except for the variant H3.3 which is cell cycle-independent. Histone H3 can be modified by phosphorylation, acetylation, ubiquitination, ribosylation, and methylation. Histone H3 has been shown to interact with Rsk-2, MSK1, GCN5 family of HATs, HDACs, PARP, SET7/9, and CARM1.
100microl
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: This monoclonal antibody was raised against a synthetic peptide from Human Histone H3, non-methylated at Lysine-9.
Formulation: Phosphate-buffered solution.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 1 mg/ml
Storage & Handling: This antibody should be handled aseptically as it is free of preservatives such as Sodium Azide. Store this antibody undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check the vial label or CoA to find the proper storage conditions.
Application: WB - Quality tested IHC-P - Verified
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1.0 - 5.0 µg per ml. For immunohistochemistry on formalin-fixed paraffin-embedded tissues, a concentration range of 5.0 - 10 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
RRID: AB_2564795 (BioLegend Cat. No. 815701)
Structure: Expected MW: 17 kD
Biology Area: Cell Biology, Neuroscience, Signal Transduction
Gene ID: 8350
UniProt: View information about Histone H3 on UniProt.org
Clone: 9B1-2G6
Regulatory Status: RUO
Other Names: Histone H3.1, histone H3/a, histone 1, H3/a, H3 histone family, member A
Previously: Covance Catalog# MMS-5083
Isotype: Mouse IgG1, κ