Biolegend, 818003, Alexa Fluor® 594 anti-Tyrosine Hydroxylase Antibody, 25μg

Tyrosine hydroxylase, also known as tyrosine 3-monooxygenase, is the enzyme that converts amino acid L-Tyrosine to L-3, 4-Dihydroxyphenylalanine (L-DOPA).  L-DOPA is a precursor to dopamine, which is then converted to norepinephrine and epinephrine (adrenaline).  Dopamine, norepinephrine and epinephrine are all important neurotransmitters.  Alterations in the activity of tyrosine hydroxylase may be involved in a number of neurological diseases such as Parkinson's and schizophrenia.
25μg
Verified Reactivity: Human, Mouse, Rat
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: This monoclonal antibody was raised against purified Tyrosine Hydroxylase from rat Pheochromocytoma tumor.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 594 under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: IHC-P - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 0.2 - 1 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 594 has an excitation maximum of 590 nm, and a maximum emission of 617 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses
Application Notes: This antibody is effective in immunoblotting (WB) and formalin-fixed paraffin-embedded immunohistochemical staining (IHC-P).This antibody is less reactive in human tissue in Western Blot.
Product Citations: Katolikova NV, et al. 2023. Int J Mol Sci. 24:. PubMed Joyce JD, et al. 2024. Int J Mol Sci. :25. PubMed
RRID: AB_2801150 (BioLegend Cat. No. 818003) AB_2801151 (BioLegend Cat. No. 818004)
Structure: Expected MW: 65 kD
Cell Type: Dopaminergic Neurons, Neural Stem Cells, Neurons
Biology Area: Cell Biology, Neuroscience, Neuroscience Cell Markers, Stem Cells, Synaptic Biology
Molecular Family: Enzymes and Regulators, Presynaptic proteins
Gene ID: 25085
UniProt: View information about Tyrosine Hydroxylase on UniProt.org
Clone: 2/40/15
Regulatory Status: RUO
Other Names: Tyrosine 3-monooxygenase, tyrosine 3-hydroxylase
Isotype: Mouse IgG2a, κ