Biolegend, 819405, Alexa Fluor® 594 anti-Histone H3 (C-terminus) Antibody, 25μg

Histone proteins are classified into core histones (H2A, H2B, H3, H4) and linker histones (H1, H5). Core histones form an octamer, which contains two H2A-H2B dimers and one H3-H4 tetramer. Core histones are predominantly globular except for the unstructured N-terminal tails. Posttranslational modifications, such as acetylation, methylation, phosphorylation, ubiquitination, SUMOylation and ADP-ribosylation occur in histone tails. Histone modifications induce changes of chromatin structure and thereby affect the accessibility of transcription factors, nuclear proteins and enzymes to genomic DNA, resulting in gene activation or repression. It is known that histone modifications play critical roles in DNA repair, DNA replication, transcription regulation, alternative splicing and chromosome condensation and some diseases including autoimmune diseases and cancers.
25μg
Verified Reactivity: Human, Mouse, Rat, Drosophila
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: This monoclonal antibody was raised against a peptide sequence derived from the C-terminal domain of human Histone H3 protein.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide; may contain stabilizer.
Preparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 594 under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: ICC - Quality tested IHC-F, IHC-P - Verified
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescence staining. For immunofluorescence microscopy, a concentration range of 1.25 - 5 μg/ml is recommended. For immunohistochemical staining on frozen tissue sections, the suggested use of this reagent is 2.5 - 10 µg per ml. For immunohistochemical staining on formalin-fixed paraffin-embedded tissue sections, the suggested use of this reagent is 1.25 - 5 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 594 has an excitation maximum of 590 nm, and a maximum emission of 617 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses
Excitation Laser: Red Laser (633 nm)
Application Notes: Reactivity to Drosophila was only verified with the purified format.
RRID: AB_2715800 (BioLegend Cat. No. 819405) AB_2715803 (BioLegend Cat. No. 819406)
Biology Area: Cell Biology, Chromatin Remodeling/Epigenetics, Neuroscience, Transcription Factors
Gene ID: 8350
UniProt: View information about Histone H3 on UniProt.org
Clone: 1B1-B2
Regulatory Status: RUO
Other Names: Histone H3.1, histone H3/a, histone 1, H3a, H3 histone family, member A, H3/A, H3FA
Isotype: Mouse IgG3, κ