Biolegend, 822501, Purified anti-MAP2 Antibody (Previously Covance catalog# PCK-554P), 50microl

Microtubules are 25nm diameter protein rods found in most kinds of eukaryote cells. They are polymerized from a dimeric subunit made of one α subunit and one β tubulin subunit. Microtubules are associated with a family of proteins called microtubule associated proteins (MAPs), which includes the protein τ (tau) and a group of proteins referred to as MAP1, MAP2, MAP3, MAP4 and MAP5. MAP2 is made up of two ~280kD apparent molecular weight bands referred to as MAP2a and MAP2b. A third lower molecular weight form, usually called MAP2c, corresponds to a pair of protein bands running at ~70 kD on SDS-PAGE gels. All these MAP2 forms are derived from a single gene by alternative transcription, and all share a C-terminal sequence which includes either three or four microtubule binding peptide sequences, which are very similar to those found in the related microtubule binding protein τ (tau). MAP2 isoforms are expressed only in neuronal cells and specifically in the perikarya and dendrites of these cells. Antibodies to MAP2 are therefore excellent markers on neuronal cells, their perikarya and neuronal dendrites. In contrast τ (tau) is found predominantly in neuronal axons.
50microl
Verified Reactivity: Mouse
Reported Reactivity: Human, Rat, Cow
Antibody Type: Polyclonal
Host Species: Chicken
Immunogen: This antibody was made against MAP2 in chickens.
Preparation: The antibody was purified by affinity chromatography.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check your vial or your CoA to find the most accurate storage condition for this antibody.
Application: IHC-P - Quality tested WB
Recommended Usage: Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. The optimal working dilution should be determined for each specific assay condition. • WB: 1:100,000* • IF: 1:10,000
Application Notes: This antibody is effective in immunoblotting (WB) and immunofluorescence (IF). This antibody does not bind to protein A or protein G. *Predicted MW = 280 kD and a doublet ~70 kD
Application References(PubMed link indicates BioLegend citation): Savas JN, et al. 2010. J Biol Chem. 285:13142-53. [IF] PubMed
Product Citations: Bartoll A, et al. 2020. EMBO Mol Med. 12:e11776. PubMed Black HH, et al. 2023. Elife. 12:. PubMed Gaudioso A, et al. 2023. Cell Death Dis. 14:248. PubMed Carus-Cadavieco M, et al. 2023. Life Sci Alliance. 6:. PubMed Liu PP, et al. 2022. EMBO Mol Med. 14:e15795. PubMed Cataldi GG, et al. 2023. J Comp Neurol. 531:486. PubMed Kodani Y, et al. 2022. Front Endocrinol (Lausanne). 13:941166. PubMed Lin YT et al. 2018. Neuron. 98(6):1141-1154 . PubMed Bresciani A, et al. 2018. PLoS One. 9:306. PubMed van Ineveld RL, et al. 2022. Nat Protoc. 17:3028. PubMed Tang QY, et al. 2020. Stem Cell Reports. 0.929861111. PubMed Ka M, Moffat J, Kim W 2016. Cereb Cortex. 10.1093/cercor/bhw319. PubMed Raja WK, et al. 2022. PLoS One. 17:e0277532. PubMed Savas J, et al. 2010. J Biol Chem. 285:13142-13153. PubMed Cui H, et al. 2022. STAR Protoc. 3:101813. PubMed Sepehrimanesh M, et al. 2021. STAR Protoc. 2:100917. PubMed Fischer M, et al. 2021. Alcohol Clin Exp Res. 45:979. PubMed Merlini M et al. 2019. Neuron. 101(6):1099-1108 . PubMed Gomez-Sintes R, et al. 2022. Nat Commun. 13:4220. PubMed Castagnola S, et al. 2020. Genome Res. 1633:30. PubMed Almansa D, et al. 2022. Int J Mol Sci. 23:. PubMed Sathler MF, et al. 2021. J Cell Sci. 134:. PubMed
RRID: AB_2564858 (BioLegend Cat. No. 822501)
Cell Type: Neural Stem Cells
Biology Area: Cell Biology, Neuroscience, Neuroscience Cell Markers, Stem Cells
Molecular Family: Microtubules
Gene ID: 4133
UniProt: View information about MAP2 on UniProt.org
Clone: Poly28225
Regulatory Status: RUO
Other Names: Microtubule-associated protein 2, MAP-2
Previously: Covance Catalog# PCK-554P
Isotype: Chicken IgY