Biolegend, 824002, Purified anti-CX3CR1 Antibody (Previously Covance catalog# MMS-5067), 25microl

CX3CR1 is a G-protein-coupled seven-transmembrane chemokine receptor, also called GPR13 or V28. It is expressed on NK cells, T cell subset, monocytes/macrophages, dendritic cells, some malignant epithelial cells, astrocytes, and microglial cells. CX3CL1 (known also as fractalkine and neurotactin) is the ligand of CX3CR1. CX3CL1 is a unique transmembrane molecule with a CX3C-motif chemokine domain and a mucin-like stalk. CX3CL1 is expressed by activated-endothelial cells, neurons, and astrocytes. The interaction of CX3CR1 and its ligand mediates cell adhesion and migration. CX3CL1 has been reported to play an important role in neuroinflammation and neurodegeneration.
25microl
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: This monoclonal antibody was raised against a peptide sequence conjugated to KLH corresponding to amino acids 207-222 of the human CX3CR1 protein.
Formulation: Phosphate-buffered solution.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 1.0 mg/ml
Storage & Handling: This antibody should be handled aseptically as it is free of preservatives such as Sodium Azide. Store this antibody undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check the vial label or CoA to find the proper storage conditions.
Application: IHC-P - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 10.0 - 20.0 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: This antibody is effective in formalin-fixed paraffin-embedded immunohistochemical staining (IHC-P).
Product Citations: Zhan L, et al. 2024. Cell Commun Signal. 457:22. PubMed Pal B, et al. 2021. EMBO J. 40:e107333. PubMed Ahn JH, et al. 2019. Int J Mol Med. 44:939. PubMed Taylor JG, et al. 2022. Haematologica. :. PubMed
RRID: AB_2734592 (BioLegend Cat. No. 824002) AB_2564876 (BioLegend Cat. No. 824001)
Structure: Expected MW: 50 kD
Biology Area: Cell Biology, Neuroinflammation, Neuroscience, Neuroscience Cell Markers
Molecular Family: Cytokine/Chemokine Receptors, GPCR
Gene ID: 1524
UniProt: View information about CX3CR1 on UniProt.org
Clone: 8E10.D9
Regulatory Status: RUO
Other Names: CX3C chemokine receptor 1, fractalkine receptor, beta chemokine receptor-like 1, chemokine (C-C) receptor-like 1, GRP13, V28
Previously: Covance Catalog# MMS-5067
Isotype: Mouse IgG1, λ