Biolegend, 826901, Purified anti-LRP2 Antibody (Previously Covance catalog# MMS-5120), 100microl

Megalin or low density lipoprotein-related protein 2 (LRP2), is a multi-ligand endocytic receptor that is expressed in many different tissues but primarily in absorptive epithelial tissues such as the kidney. LRP2 mediates uptake of Apo-J which is a binding protein for Amyloid beta. Megalin has a large amino-terminal extracellular domain, a single transmembrane domain, and a short carboxy-terminal cytoplasmic tail. The extracellular ligand-binding-domains bind diverse macromolecules including albumin, apolipoproteins B and E, and lipoprotein lipase. Megalin is critical for the reuptake of numerous ligands, including lipoproteins, sterols, vitamin-binding proteins, and hormones. It also has a role in cell-signaling; extracellular ligands include parathyroid hormones and the morphogen sonic hedgehog while cytosolic ligands include MAP kinase scaffold proteins and JNK interacting proteins. Recycling of this membrane receptor is regulated by phosphorylation of its cytoplasmic domain. Mutations in the LRP2 gene cause Donnai-Barrow syndrome (DBS) and facio-oculoacoustico-renal syndrome (FOAR).
100microl
Verified Reactivity: Mouse, Rat
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: This monoclonal antibody was raised against purified human LRP2 protein.
Formulation: Phosphate-buffered solution.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 1 mg/ml
Storage & Handling: This antibody should be handled aseptically as it is free of preservatives such as Sodium Azide. Store this antibody undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check the vial label or CoA to find the proper storage conditions.
Application: IHC-P - Quality tested IHC-F - Reported in the literature, not verified in house
Recommended Usage: Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 5.0 - 10 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: This antibody is effective in immunohistochemistry (IHC-P and IHC-F).
Application References(PubMed link indicates BioLegend citation): Nashida T, et al. 2013. Arch. Biochem. Biophys. 530(1)32-9. (IHC-F)
RRID: AB_2564906 (BioLegend Cat. No. 826901)
Biology Area: Cell Biology, Neuroinflammation, Neuroscience
Molecular Family: Apolipoproteins
Gene ID: 1472529216
UniProt: View information about LRP2 on UniProt.org
Clone: CD7D5
Regulatory Status: RUO
Other Names: Low-density lipoprotein receptor-related protein 2, LRP-2, megalin, glycoprotein 330, Heymann nephritis antigen homolog, calcium sensor protein
Previously: Covance Catalog# MMS-5120
Isotype: Mouse IgG1