Biolegend, 828101, Purified anti-MAP2 Antibody (Previously Covance catalog# MMS-485P), 100microl

Microtubules are required for many well characterized functions in eukaryotic cells, including the movement of chromosomes in mitosis and meiosis, intracellular transport, establishment and maintenance of cellular morphology, cell growth, cell migration and morphogenesis in multicellular organisms. Microtubules are associated with a family of proteins called microtubule associated proteins (MAPs), which includes the protein t (tau) and a group of proteins referred to as MAP1, MAP2, MAP3, MAP4 and MAP5. MAP2 is made up of two ~280kDa apparent molecular weight bands referred to as MAP2a and MAP2b. A third lower molecular weight form, usually called MAP2c, corresponds to a pair of protein bands running at ~70kDa on SDS-PAGE gels. All these MAP2 forms are derived from a single gene by alternate transcription.
100microl
Verified Reactivity: Human, Mouse
Reported Reactivity: Other species
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: Phosphate-buffered solution + 0.03% thimerosal.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 1 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check your vial or your CoA to find the most accurate storage condition for this antibody.
Application: WB
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. The optimal working dilution should be determined for each specific assay condition. • Western blot: 1:1,000*
Application Notes: This antibody is effective in immunoblotting. *Predicted MW = 280 kD This antibody will stain neuronal cell bodies and dendritic processes, and will not stain glial cells or axons. It is the best marker to distinguish dendrites from axons in the brain.
Application References(PubMed link indicates BioLegend citation): Olmsted JB. Microtubule-associated proteins. Annu Rev Cell Biol 2:421-57, 1986. Matus A. Microtubule associated proteins: Their potential role in determining neuronal morphology. Annu Rev Neurosci 11:29-44, 1988.
RRID: AB_2564917 (BioLegend Cat. No. 828101)
Cell Type: Neural Stem Cells
Biology Area: Cell Biology, Neuroscience, Neuroscience Cell Markers, Stem Cells
Molecular Family: Microtubules
Gene ID: 4133
UniProt: View information about MAP2 on UniProt.org
Clone: AA6
Regulatory Status: RUO
Other Names: Microtubule-associated protein 2, MAP-2
Previously: Covance Catalog# MMS-485P
Isotype: Mouse IgG1, κ