Biolegend, 828301, Purified anti-p120 Catenin Antibody (Previously Covance catalog# MMS-5040), 100microl

p120/Catenin delta-1 (CTNND1) is a membrane protein that binds to cadherins and participates in signal transduction.  It is the prototypic member of a growing subfamily of Armadillo-domain proteins found at cell-cell junctions and in nuclei.  Known to bind specifically to presenilin 1, it regulates the maintenance of dendrites and dendritic spines in mature cortex.
100microl
Verified Reactivity: Human, Mouse
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: This monoclonal antibody was raised against tyrosine phosphoproteins purified from chicken embryonic fibroblasts (CEFs) expressing pp60/c-src 527F and 2A/527F.
Formulation: Phosphate-buffered solution.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 1 mg/ml
Storage & Handling: This antibody should be handled aseptically as it is free of preservatives such as Sodium Azide. Store this antibody undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check the vial label or CoA to find the proper storage conditions.
Application: WB - Quality testedIP - Reported in the literature, not verified in house
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 5 - 10 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.
Application References(PubMed link indicates BioLegend citation): Kanner S., et al. Tyrosine phosphorylation of a 120-kilodalton pp60src substrate upon epidermal growth factor and platelet-derived growth factor receptor stimulation and in polyomavirus middle-T-antigen-transformed cells. Mol Cell Bio, 11(2): 713-720, February 1991. Kanner S., et al. Monoclonal antibodies to individual tyrosine-phosphorylated protein substrates of oncogene-encoded tyrosine kinases. Proc Natl Acad Sci U.S.A., 87(9): 3328-3332, May 1990. Staddon JM, et al. 1995. J. Cell. Biol. 130:369. (WB, IP) Kim L and Wong TW, et al. 1995. Mol. Cell. Biol. 15:4553. (WB, IP)
Product Citations: Alharatani R, et al. 2020. Hum Mol Genet. 29:1900. PubMed
RRID: AB_2564919 (BioLegend Cat. No. 828301)
Structure: Expected MW: 115 kD
Biology Area: Cell Biology, Neuroscience, Synaptic Biology
Molecular Family: Adhesion Molecules
Gene ID: 1500
UniProt: View information about p120 Catenin on UniProt.org
Clone: 2B12
Regulatory Status: RUO
Other Names: CAS, p120, CTNND, P120CAS, P120CTN, p120(CAS), p120(CTN), catenin delta-1
Previously: Covance Catalog# MMS-5040
Isotype: Mouse IgG1, κ