Biolegend, 828601, Purified anti-IGBP-1 Antibody (Previously Covance catalog# MMS-5088), 100microl

IGBP1 (also known as Alpha4) , binds to PP2A C in the absense of A and a B subunit in a catalytically inactive state to prevent C proteosomal degradation.  If alpha4 levels are low, PP2A levels can decrease, leading to increased tau protein phosphorylation and aggregation. Protein phosphatase 2A is a heterotrimeric protein serine/threonine phosphatase consisting of a 36-kD catalytic C subunit (alpha and beta isoforms), a 65-kD structural A subunit (alpha and beta isoforms), and a variable regulatory B subunit. When the PP2A catalytic C subunit associates with the A and B subunits several species of holoenzymes are produced with distinct functions and characteristics. Ubiquitously expressed, PP2A is involved in a broad range of cellular processes, including membrane receptor desensitization, signal transduction, intermediary metabolism, transcriptional regulation and control of DNA replication, and mitosis. The PP2A phosphatase family accounts for the majority of Ser/Thr phosphatase activity in the brain and specifically regulates tau phosphorylation, both directly as tau phosphatase and indirectly by regulating the activities of several tau kinases which include CaM Kinase II, PKA, MAP kinase kinase (MEK 1/2), extracellular regulated kinase (ERK 1/2), GSK-3β, and P70S6 kinase, thus regulating tau microtubule binding in the brain.
100microl
Verified Reactivity: Human, Mouse, Rat
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: This monoclonal antibody was raised against an N-terminal 6-HIS tagged fusion protein corresponding to full-length mouse Alpha4 protein.
Formulation: Phosphate-buffered solution.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 1 mg/ml
Storage & Handling: This antibody should be handled aseptically as it is free of preservatives such as Sodium Azide. Store this antibody undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check the vial label or CoA to find the proper storage conditions.
Application: WB - Quality testedICC, IP - Reported in the literature, not verified in house
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1.0 - 10 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.
Application References(PubMed link indicates BioLegend citation): Kong M, et al. 2009. Mol Cell. 36:51. (IP, ICC, WB)
RRID: AB_2564922 (BioLegend Cat. No. 828601)
Structure: Expected MW: 40 kD
Biology Area: Cell Biology, Neurodegeneration, Neuroscience, Protein Misfolding and Aggregation
Molecular Family: Phospho-Proteins
Gene ID: 8518
UniProt: View information about IGBP-1 on UniProt.org
Clone: 5F6
Regulatory Status: RUO
Other Names: Alpha4, Immunoglobulin-binding protein 1, CD79a-binding protein 1, phosphoprotein, component, 52 kD, lymphocyte signal transduction molecule alpha 4, ALPHA-4, IBP1, renal carcinoma antigen NY-REN-16
Previously: Covance Catalog# MMS-5088
Isotype: Mouse IgG1, κ