Biolegend, 829301, Purified anti-HSF2 Antibody (Previously Covance catalog# MRT-5114), 100microl

Heat shock factor protein 2 (HSF2) is a transcription factor that enters the nucleus and binds to heat shock factor promoter elements, consisting of a consensus tandem array sequence of three oppositely oriented "AGAAN" motifs, during heat shock in eukaryotes.  Under normal conditions, it exists as a homodimer in the cytoplasm of cells where it binds to heat shock proteins and inactivates them.  During high temperature cellular stress, misfolding of proteins occurs, which causes heat shock proteins to dissociate from the heat shock factor proteins and bind to the misfolded proteins.  This leads to free heat shock proteins, which can then enter the nucleus and activate transcription.
100microl
Verified Reactivity: Human, Mouse, Rat
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: This monoclonal antibody was raised against mouse recombinant HSF-2 protein.
Formulation: Phosphate-buffered solution.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 1 mg/ml
Storage & Handling: This antibody should be handled aseptically as it is free of preservatives such as Sodium Azide. Store this antibody undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check the vial label or CoA to find the proper storage conditions.
Application: WB - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 1.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: This antibody is effective in immunoblotting (WB).
Application References(PubMed link indicates BioLegend citation): Sistonen L, et al. 1992. Mol Cell Biol. 12(9): 4104-11.
RRID: AB_2564928 (BioLegend Cat. No. 829301)
Structure: Expected MW: 70 kD
Biology Area: Cell Biology, Neurodegeneration, Neuroscience, Protein Trafficking and Clearance
Molecular Family: Heat Shock Proteins
Gene ID: 3298
UniProt: View information about HSF2 on UniProt.org
Clone: 3E2
Regulatory Status: RUO
Other Names: Heat shock factor protein 2, HSF 2, HSTF 2, heat shock transcription factor 2
Previously: Covance Catalog# MRT-5114
Isotype: Rat IgG1, κ