Biolegend, 829401, Purified anti-GFAP Antibody (Previously Covance catalog# PCK-591P), 100microl

Glial fibrillary acidic protein (GFAP) is the 10nm or intermediate filament protein found specifically in astrocytic cells in the central nervous system. Antibodies to GFAP are therefore widely used to identify astrocytic cells in situ and in tissue culture. This antibody was developed using bovine spinal cord GFAP purified from a Triton X-100 extract of myelin associated material and further purified by centrifugation and ion exchange chromatography in 6m urea on DEAE cellulose.
100microl
Verified Reactivity: Human, Mouse, Rat
Antibody Type: Polyclonal
Host Species: Chicken
Immunogen: Native GFAP purified from bovine spinal cord.
Formulation: Phosphate-buffered solution, containing 0.2% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check your vial or your CoA to find the most accurate storage condition for this antibody.
Application: IHC-P - Quality testedWB - Verified
Recommended Usage: Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a dilution range of 1:1000 - 1:5000 is suggested. For Western blotting, the suggested use of this reagent is a 1:5000 - 1:10000 dilution. It is recommended that the reagent be titrated for optimal performance for each application.
Application References(PubMed link indicates BioLegend citation): Sosunov AA, et al. 2013. J. Neurosci. 33:7439-50. (IHC, WB) PubMed
Product Citations: Iguchi A, et al. 2023. iScience. 26:106375. PubMed Zhang R, et al. 2023. Stroke Vasc Neurol. . PubMed McNamara NB, et al. 2023. Nature. 613:120. PubMed Nam HS, et al. 2023. Neural Dev. 18:1. PubMed Jang MJ, et al. 2023. Nat Biotechnol. :. PubMed Chen S, et al. 2022. Acta Neuropathol. 143:547. PubMed Proulx J, et al. 2022. Front Neurosci. 16:906651. PubMed Sosunov A, et al. 2013. J Neurosci. 33:7439-7450. PubMed Li J, et al. 2019. Glia. 67:1571. PubMed Gómez-Pinedo U, et al. 2021. Int J Mol Sci. 22: . PubMed Guttikonda SR, et al. 2021. Nat Neurosci. 24:343. PubMed Moezzi D, et al. 2022. Sci Rep. 12:12761. PubMed Banitalebi S, et al. 2022. Int J Mol Sci. 23:. PubMed Gangoso E, et al. 2021. Cell. 184:2454. PubMed Yang S, et al. 2022. Mol Psychiatry. :. PubMed Lozinski BM, et al. 2021. Sci Rep. 11:7239. PubMed Rao SB, et al. 2021. Biochimica et Biophysica Acta (BBA) - Biomembranes. 1863(8):183616. PubMed Datta D, et al. 2021. Front Aging Neurosci. 13:760270. PubMed
RRID: AB_2564929 (BioLegend Cat. No. 829401)
Structure: GFAP is a 432 amino acid protein with a molecular mass of ~50 kD.
Distribution: Tissue distribution: GFAP is expressed by numerous cell types of the central nervous system (CNS) including astrocytes, ependymal cells, and Bergmann glia cells (protoplasmic astrocyte). GFAP is expressed in cells lacking fibronectin. Cellular distribution: Cytoskeleton and cytosol Function GFAP is a class-III intermediate filament and a structural constituent of the cytoskeleton. It is a cell-specific marker that is used to distinguish astrocytes from other glial cells during the development of the CNS.
Cell Type: Astrocytes
Biology Area: Cell Biology, Neuroscience, Neuroscience Cell Markers
Molecular Family: Intermediate Filaments
Gene ID: 2670
UniProt: View information about GFAP on UniProt.org
Clone: Poly28294
Regulatory Status: RUO
Other Names: Glial fibrillary acidic protein
Previously: Covance Catalog# PCK-591P
Isotype: Chicken IgY