Biolegend, 829901, Purified anti-TDP43 Phospho (Ser409/410) Antibody (Previously Covance catalog# SIG-39852), 100microl

TDP43 (aka TARDBP) was originally identified as a protein which binds to the transactivation response (TAR) sequence found in the long terminal repeat of the HIV-1 virus genome. TDP43 contains two copies of a ~90 amino acid RNA recognition motif (RRM) found in many proteins which bind single stranded RNA. TDP-43 localizes to the nucleus. In patients with frontotemporal lobar degeneration and amyotrophic lateral sclerosis, it is absent from the nucleus of affected neurons but it is the primary component of cytoplasmic ubiquitin-positive inclusion bodies.  Ubiquitously expressed. In particular, expression is high in pancreas, placenta, lung, genital tract and spleen. Hyperphosphorylated, ubiquitinated, and cleaved in hippocampus, neocortex, and spinal cord from individuals affected with ALS and FTLDU.
100microl
Verified Reactivity: Human, Rat
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: This antibody was raised against a phosphopeptide corresponding to: i) the C-terminal domain of human TDP43; and ii) inclusive of phosphorylated serine residues 409 and 410.
Formulation: Phosphate-buffered solution.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 1.0 mg/mL
Storage & Handling: This antibody should be handled aseptically as it is free of preservatives such as Sodium Azide. Store this antibody undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check the vial label or CoA to find the proper storage conditions.
Application: WB - Quality tested IHC, ICC, ELISA- Reported in the literature, not verified in house
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. The optimal working dilution should be determined for each specific assay condition. • WB: 1:1,000
Application Notes: This antibody is effective in immunoblotting (WB) Positive Control: Rat Brain nuclear lysate Expected MW: ~43 kD
Application References(PubMed link indicates BioLegend citation): Neumann M, et al. 2009. Acta Neuropathol. 117:137-49. Sarroukh R, et al. 2011. Cell Mol Life Sci. 68:1429. (WB, IHC, ELISA, IF) Ramakrishnan M, et al. 2009. Biochemistry 48:10405. (WB) Watts JC, et al. 2014. Proc. Natl. Acad. Sci. USA. 111:10323. (WB, IHC) Diomede L, et al. 2014. Neurobiol Dis. 62:521. (IHC)
Product Citations: Keating SS, et al. 2023. Cell Mol Life Sci. 80:95. PubMed Melikyan ZA, et al. 2022. Neurobiol Aging. 116:12. PubMed Ackermans NL, et al. 2022. Acta Neuropathol. 144:5. PubMed Latimer CS, et al. 2022. Methods Mol Biol. 2561:3. PubMed Gasset-Rosa F, et al. 2019. Neuron. 102:339. PubMed Gleixner AM, et al. 2022. Nat Commun. 13:3380. PubMed Liu EA, et al. 2021. Neuropathol Appl Neurobiol. 47:1019. PubMed Wiesner D, et al. 2017. Exp Neurol. 10.1016/j.expneurol.2017.09.011. PubMed Wani A, et al. 2021. Cell Reports. 36(3):109399. PubMed
RRID: AB_2564934 (BioLegend Cat. No. 829901)
Biology Area: Cell Biology, Neurodegeneration, Neuroscience, Protein Misfolding and Aggregation
Molecular Family: Phospho-Proteins, TDP43
Gene ID: 23435298648
UniProt: View information about TDP43 Phospho Ser409/410 on UniProt.org
Clone: 1D3/TDP-43
Regulatory Status: RUO
Other Names: TAR DNA-binding protein-43, TDP 43, TARDBP, ALS10
Previously: Covance Catalog# SIG-39852
Isotype: Rat IgG2a