Biolegend, 831301, Purified anti-PPP2R2A Antibody (Previously Covance catalog# MMS-5099), 100microl

Protein phosphatase 2A is a heterotrimeric protein serine/threonine phosphatase consisting of a 36-kD catalytic C subunit (alpha and beta isoforms), a 65-kD structural A subunit (alpha and beta isoforms), and a variable regulatory B subunit. When the PP2A catalytic C subunit associates with the A and B subunits several species of holoenzymes are produced with distinct functions and characteristics. Ubiquitously expressed, PP2A is involved in a broad range of cellular processes, including membrane receptor desensitization, signal transduction, intermediary metabolism, transcriptional regulation and control of DNA replication, and mitosis. The PP2A phosphatase family accounts for the majority of Ser/Thr phosphatase activity in the brain and specifically regulates tau phosphorylation, both directly as tau phosphatase and indirectly by regulating the activities of several tau kinases which include CaM Kinase II, PKA, MAP kinase kinase (MEK 1/2), extracellular regulated kinase (ERK 1/2), GSK-3β, and P70S6 kinase, thus regulating tau microtubule binding in the brain.
100microl
Verified Reactivity: Human, Mouse, Rat
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: This monoclonal antibody was raised against a synthetic peptide corresponding to residues surrounding Asp406 of rat PP2R2A/PP2A, B55-α/PR55-α protein.
Formulation: Phosphate-buffered solution with BSA + 0.09% NaN3.Previous lots of this product may have been formulated with 0.1% or 0.05% NaN3 instead of 0.09% NaN3. For further information please contact BioLegend Technical Support or Customer Service.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 1 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check your vial or your CoA to find the most accurate storage condition for this antibody.
Application: WB - Quality testedIHC-P - Reported in the literature, not verified in house
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 10 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.
Application References(PubMed link indicates BioLegend citation): Sontag E, et al. 2004. J Neuropathol Exp Neurol. 63:287. (WB, IHC-P)
RRID: AB_2564946 (BioLegend Cat. No. 831301)
Structure: Expected MW: 55 kD
Cell Type: Neurons
Biology Area: Cell Biology, Neurodegeneration, Neuroscience, Neuroscience Cell Markers, Protein Misfolding and Aggregation
Molecular Family: Protein Kinases/Phosphatase
Gene ID: 5520
UniProt: View information about PPP2R2A on UniProt.org
Clone: 2G9
Regulatory Status: RUO
Other Names: Serine/threonine-protein phosphatase 2A 55 kD regulatory subunit B alpha isoform, B55A, B55ALPHA, PR52A, PR55A, protein phosphatase 2 (formerly 2A), regulatory subunit B (PR52)
Previously: Covance Catalog# MMS-5099
Isotype: Mouse IgG1