Biolegend, 834402, Purified anti-HSD17B10 Antibody (Previously Covance catalog# SIG-39770), 500microl

The clone 5F3 recognizes the endogenous HSD17B10 protein in extracts from cells with virtually no cross-reactivity to other human proteins in immunoblots and ELISA assays. HSD17B10 is an intracellular 27kD polypeptide which appears to be up regulated in the brains of Alzheimer’s disease patients and has been associated with amyloid beta neurotoxicity. Cross-reactivity to mouse or other species has not been tested.
500microl
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: This antibody was generated against recombinant, full-length human HSD17B10 protein.
Formulation: Phosphate-buffered solution; no preservatives or carrier proteins.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 1 mg/ml
Storage & Handling: This antibody should be handled aseptically as it is free of preservatives such as Sodium Azide. Store this antibody undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check the vial label or CoA to find the proper storage conditions.
Application: IHC-P - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 1.0 - 10 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: Clone 5F3 recognizes the recombinant human HSD17B10 protein (27kD) and is an excellent mitochondrial marker.Please note that the name of this product has changed from Purified anti-ERAB Antibody to Purified anti-HSD17B10 Antibody.
Application References(PubMed link indicates BioLegend citation): He XY, et al. 2000. Biochim BioPhys Acta. 1484 (2-3):267-77. Yan SD, et al. 1999. J Biol Chem. 274(4):2145-56. Oppermann UC, et al. 1999. FEBS Lett. 451(3):238-42. Sambamurti K & Lahiri DK. 1998. Biochem Biophys Res Commun. 249(2):546-9. Yan SD, et al. 1997. Nature. 389(6652):689-95. Furuta S, et al. 1997. Biochim BioPhys Acta. 1350(3):317-24.
RRID: AB_2783409 (BioLegend Cat. No. 834405) AB_2783408 (BioLegend Cat. No. 834404) AB_2564990 (BioLegend Cat. No. 834402)
Biology Area: Cell Biology, Mitochondrial Function, Neuroscience, Neuroscience Cell Markers, Signal Transduction
Molecular Family: Mitochondrial Markers
Gene ID: 3028
UniProt: View information about HSD17B10 on UniProt.org
Clone: 5F3
Regulatory Status: RUO
Other Names: 17b-HSD10, ABAD, CAMR, DUPXp11.22, HADH2, HCD2, MHBD, MRPP2, MRX17, MRX31, MRXS10, SCHAD, SDR5C1, HSD17B10, HSD-10, 3-hydroxyacyl-CoA dehydrogenase type-2, AB-binding alcohol dehydrogenase, mitochondrial ribonuclease P protein 2
Previously: Signet Catalog# 9770-02Signet Catalog# 9770-05Signet Catalog# 9770-10Covance Catalog# SIG-39770
Isotype: Mouse IgG1, λ