Biolegend, 834701, Purified anti-FMRP Antibody (Previously Covance catalog# MMS-5232), 100microl

The gene encoding Fragile X mental retardation protein 1 (FMRP or FMR1), also known as premature ovarian failure 1 (POF1), contains multiple CGG sequence repeats that are positively correlated with impaired cognitive and reproductive function. FMRP may be involved in mRNA transport and synaptic protein synthesis. The gene that encodes FMRP is located in the X chromosome, and an abnormally large number of CGG repeats (200 - 1000 times) causes Fragile X syndrome due to inadequate amounts of FMRP, leading to severe learning deficiency and mental retardation.
100microl
Verified Reactivity: Human, Mouse
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: This monoclonal antibody was raised against a recombinant protein isolated from baculovirus expressing mouse FMRP.
Formulation: Phosphate-buffered solution.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 1 mg/ml
Storage & Handling: This antibody should be handled aseptically as it is free of preservatives such as Sodium Azide. Store this antibody undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check the vial label or CoA to find the proper storage conditions.
Application: WB - Quality tested ICC, IHC-P - Verified
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1.0 - 2.0 µg per ml. For immunohistochemistry on formalin-fixed paraffin-embedded tissue, a concentration range of 5.0 - 10 µg/ml is suggested. For immunocytochemistry, a concentration range of 5.0 - 10 μg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: This antibody is effective in immunoblotting (WB), immunohistochemistry on formalin-fixed paraffin-embedded tissue (IHC-P) and immunocytochemistry (ICC). The epitope for 5C2 lies between amino acids 347-353 of FMRP. This clone does not cross react with FXR1 or FXR2.
Application References(PubMed link indicates BioLegend citation): Gholizadeh S, et al. 2014. Neuropsychopharmacology. 39:3100.
Product Citations: Kalinowska M, et al. 2022. Mol Autism. 13:29. PubMed Raj N, et al. 2021. Cell Reports. 35(2):108991. PubMed Graef JD, et al. 2020. Eur J Neurosci. 51:2143. PubMed Liu XS, et al. 2018. Cell. 172:979. PubMed
RRID: AB_2750045 (BioLegend Cat. No. 834702) AB_2564993 (BioLegend Cat. No. 834701)
Structure: Expected MW: 68-80 kD (smaller truncations of FMRP seen at lower molecular weights)
Biology Area: Cell Biology, Neuroscience, Synaptic Biology, Transcription Factors
Molecular Family: Postsynaptic proteins, Presynaptic proteins
Gene ID: 2332
UniProt: View information about FMRP on UniProt.org
Clone: 5C2
Regulatory Status: RUO
Other Names: Fragile X mental retardation protein 1, POF, FMRP, POF1, FRAXA
Previously: Covance Catalog# MMS-5232
Isotype: Mouse IgG1, κ