Biolegend, 836303, Purified anti-SNAP-25 Antibody, 25μg

Synaptosomal-associated protein 25 (SNAP-25) is a component of the trans-SNARE (t-SNARE) complex. SNAP-25 has been reported to account for the specificity of membrane fusion, and to directly execute fusion by forming a tight complex that brings the synaptic vesicle and plasma membranes together. SNAP-25 has been shown to inhibits both ATP-dependent and independent Ca 2+ -triggered release of glutamate from central nervous system (CNS) synaptosomal membranes. This indicates that SNAP-25 has a role not only in the formation of the synaptic vesicle-target v- and t-SNARE complex, but also in the final neurotransmitter release.
25μg
Verified Reactivity: Human, Mouse, Rat
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Full length, unaltered SNAP-25
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: IHC-P - Quality tested WB - Verified EM, FC, ICC, IP - Reported in the literature, not verified in house
Recommended Usage: Each lot of this antibody is quality control tested by immunohistochemistry. For immunohistochemistry, a concentration range of 0.1 - 1.0 µg per ml. For Western blotting, the suggested use of this reagent is 0.5 - 5.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.
Application References(PubMed link indicates BioLegend citation): Postupna NO, et al. 2014. Lab Invest. 94:1161. (FC) Hirano AA, et al. 2011. J. Comp. Neurol. 519:972. (WB, IHC, EM) PubMed Flores-Otero J, et al. 2007. J. Neurosci. 27:14023. (IF) PubMed Rozas JL, et al. 2012. Neuron. 74:151. (IF) PubMed Brinkmalm A, et al. 2014. Mol. Neurodegener. 9:53. (IP) PubMed Gu Y, et al. 2016. Proc. Natl. Acad. Sci. U. S. A. E922-31. (WB) PubMed Kabachinski G, et al. 2016. Mol. Biol. Cell. 654-68.(IF) PubMed Kunii M, et al. 2016. J. Cell Biol. 215:121-138. (IP) PubMed
Product Citations: Wang Q, et al. 2023. Front Mol Biosci. 10:1163545. PubMed Junakovic A, et al. 2023. Brain Struct Funct. 228:613. PubMed Brinkmalm A, et al. 2014. Mol Neurodegener. 9:53. PubMed Hirano A, et al. 2011. J Comp Neurol. 519:972-988. PubMed Wang W, et al. 2021. Int J Mol Sci. 22:. PubMed Marcelli S, et al. 2019. Sci Rep. 9:7146. PubMed Gu Y, et al. 2016. Proc Natl Acad Sci U S A. 113: 922 - 931. PubMed Kandachar V, et al. 2018. J Cell Sci. 131:jcs222034. PubMed Xie YX, et al. 2022. Nat Commun. 13:4918. PubMed Konstantinović J, et al. 2018. J Med Chem. 61:1595. PubMed Stewart M, et al. 2019. Dis Model Mech. 12:. PubMed Wilhelmi I, et al. 2020. Mol Metab. 45:101151. PubMed Kabachinski G, et al. 2016. Mol Biol Cell. 27: 654 - 668. PubMed Flores-Otero J, et al. 2007. J Neurosci. 27:14023-14034. PubMed Kunii M, et al. 2016. J Cell Biol. 215: 121 - 138. PubMed
RRID: AB_2715864 (BioLegend Cat. No. 836303) AB_2566521 (BioLegend Cat. No. 836304)
Structure: SNAP-25 is a 206 amino acid protein with a molecular mass of 23 kD.
Distribution: Tissue distribution: Mainly in the central nervous system, in neurons of the neocortex, hippocampus, piriform cortex, anterior thalamic nuclei, pontine nuclei, and granule cells of the cerebellum Cellular distribution: Cytosol, golgi apparatus, and cell membrane
Function: SNAP-25 associates with proteins involved in vesicle docking and membrane fusion. It regulates plasma membrane recycling through its interaction with centromere protein F (CENPF).
Interaction: In adult tissue, SMI 81 visualizes synapse-rich areas of brain and retina. In developing brain and cell cultures, SMI 81 reacts with neuronal cell bodies and axons. It is useful as an early marker for developing neurons in cell culture.
Cell Type: Neurons
Biology Area: Cell Biology, Neuroscience, Signal Transduction, Synaptic Biology
Molecular Family: Presynaptic proteins
Antigen References: 1. Snyder DA, et al. 2006. Cell Biochem. Biophys. 45(1):111-23. 2. Nagy G, et al. 2004. Neuron. 41(3):417–29.
Gene ID: 6616
UniProt: View information about SNAP-25 on UniProt.org
Clone: SMI 81
Regulatory Status: RUO
Other Names: Synaptosomal-associated protein 25, SUP, Synaptosomal-associated 25 kD Protein
Isotype: Mouse IgG1, κ