Biolegend, 836504, Purified anti-Myelin Basic Protein Antibody, 100μg

Myelin basic protein (MBP) is a protein involved in the myelination of nerves in the central nervous system (CNS). MBP functions to maintain the correct structure of myelin, through its interaction with the lipids in the myelin membrane. The myelin sheath is made up of MBP and lipids, and acts as an insulator to increase the velocity of axonal impulse conduction. MBP plays a central role in the demyelinating disease multiple sclerosis (MS). A hallmark of the disease is the loss of the myelin sheath surrounding nerves, thought to be induced by antibodies against MBP.
100μg
Verified Reactivity: Human, Mouse, Rat
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: IHC-P - Quality tested WB - Verified ICC, IHC-F - Reported in the literature, not verified in house
Recommended Usage: Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemcical staining. For immunohistochemistry, a concentration range of 1.0 - 5.0 μg/ml is suggested. For Western blotting, the suggested use of this reagent is 1.0 - 5.0 µg/ml. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: SMI 94 reacts with the Myelin Basic Protein (MBP) peptic fragment 70-89 of the classic human myelin basic protein sequence. SMI 94 detects developing and adult myelin, developing oligodendrocytes and distinguishes oligodendrocytes from astrocytes, microglia, neurons and other cells in brain sections. The combination of SMI 94 with SMI 91 and/or 99 is useful for immunocytochemical studies on the progression of normal and pathologic myelination.
Application References(PubMed link indicates BioLegend citation): Kiryu-Seo S, et al. 2010. J. Neurosci. 30:6658. (ICC) Gensel JC, et al. 2009. J. Neurosci. 29:3956. (IHC-P) Wang H, et al. 2008. J. Cell. Biol. 182:1171. (ICC, WB) McLean NA, et al. 2014. PLoS One 9:e110174. (IHC-F) Tian C, et al. 2014. Front Cell Neurosci. 8:297. (IHC-P) Wüthrich C, et al. 2009. J. Neuropathol. Exp. Neurol. 68:15. (IHC, ICC) Maire CL, et al. 2014. Stem Cells 32:313. (IHC-P) Rusielewicz T, et. al. 2014. Glia 62:580. (ICC) Li C, et al. 2012. ASN Neuro. 4:e00102. (ICC) Huang JH, et al. 2009. Tissue Eng. Part A 15:1677. (IHC-P) Göttle P, et al. 2015. J. Neurosci. 35:906. (ICC) Zhao X, et al. 2010. Neuron. 65:612. (ICC)
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RRID: AB_2616694 (BioLegend Cat. No. 836504)
Structure: Myelin Basic Protein is a 304 amino acid protein with a molecular mass of 33kD.
Distribution: Cytoskeleton, plasma membrane, cytosol, and extracellular.
Function: Classic MBP isoforms (4-14) are the most abundant protein components of the myelin membrane in the CNS. They play a role in both its formation and stabilization. The non-classic isoforms may have a role in the early developing brain long before myelination.
Biology Area: Cell Biology, Neuroscience, Neuroscience Cell Markers
Gene ID: 4155
UniProt: View information about Myelin Basic Protein on UniProt.org
Clone: SMI 94
Regulatory Status: RUO
Other Names: Golli-MBP, myelin A1 protein, myelin membrane encephalitogenic protein
Isotype: Mouse IgG1, κ