Biolegend, 836804, Purified anti-Rat Blood-Brain Barrier Antibody, 100μg

SMI 71 is specific for a rat endothelial protein found in areas with blood-brain or blood-nerve barriers. The antibody does not react with endothelia of periventricular organs or with fenestrated endothelia in peripheral tissues. Specifically, it has been shown that staining with SMI 71 is not observed in blood vessels and sinusoids in the liver, vessels in the heart, adrenal, skeletal muscle, intestine, thymus, lymph nodes, pancreas, thyroid, or skin. However, a patchy reaction was observed on some vessel walls of the spleen and epidermis of the skin 3 . Reactivity with the antibody develops in newborn rats along with maturation of the blood brain barrier. Reactivity disappears in lesions of experimental allergic encephalomyelitis. Contrary to the belief that astrocytes and perhaps neurons are essential for the establishment of the blood-brain barrier, destruction of neurons (monitored by SMI 311 and anti-MAP2) and of astrocytes (monitored by anti-GFAP and anti-S100B) leads only to transient abolition of SMI 71 reactivity and only transient transendothelial passage of serum albumin.
100μg
Verified Reactivity: Rat
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: IHC-P - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 5.0 - 10.0 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
Application References(PubMed link indicates BioLegend citation): Ghabriel MN, et al. 2002. Reproduction 123:389-97. (IHC) Beaumont M, et. al. 2009. J. Cereb. Blood Flow Metab. 29:1714. (IHC) PubMed Sternberger NH, Sternberger LA. 1987. Proc. Natl. Acad. Sci. USA 84(22):8169. (IHC) PubMed
Product Citations: Rigollet S, et al. 2024. Theranostics. 4147:14. PubMed Zhang L, et al. 2022. Front Aging Neurosci. 14:926485. PubMed Hubbard WB, et al. 2022. Acta Neuropathol Commun. 10:93. PubMed
RRID: AB_2687236 (BioLegend Cat. No. 836803) AB_2687237 (BioLegend Cat. No. 836804)
Biology Area: Cell Biology, Neuroscience, Neuroscience Cell Markers
Gene ID: NA
UniProt: View information about Blood-Brain Barrier on UniProt.org
Clone: SMI 71
Regulatory Status: RUO
Other Names: Endothelial barrier antigen, EBA
Isotype: Mouse IgM, κ