Biolegend, 838004, Purified anti-O-GlcNAc Antibody, 100μg

β-O-linked N-acetylglucosamine (O-GlcNAc) is an abundant post-translational modification occuring on serine and threonine residues. The modification has been detected on a variety of nuclear and cytoplasmic proteins involved in diverse cellular functions. Studies to elucidate the role of O-GlcNAc-ylation have been hampered by the lack of a sensitive, specific assay to identify or purify proteins bearing O-GlcNAc.
100μg
Verified Reactivity: All Species
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: The antibody was raised against a synthetic peptide containing Serine-O-GlcNAc.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested ICC, IHC-P - Verified IP - Reported in the literature, not verified in house
Recommended Usage: Each lot of this antibody is quality control tested by western blotting. For immunocytochemistry, a concentration range of 2.5 - 10 μg/mL is recommended. For immunohistochemical staining on formalin-fixed paraffin-embedded tissue sections, the suggested use of this reagent is 1.0 - 10 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: This antibody detects Ser-O-GlcNAc and Thr-O-GlcNAc but shows no cross-reactivity with peptide determinants or other closely-related carbohydrate antigens. Interaction between the antibody and the Ser- or Thr-O-GlcNAc epitope can be blocked by competition with free GlcNAc. Milk proteins may cross-react with the antibody therefore we recommend using BSA or goat serum as a blocker. Additional reported applications (for the relevant formats) include: ELISA2, immunocytochemistry7, immunoprecipitation2,5,6, and Western blotting3,4,7.
Application References(PubMed link indicates BioLegend citation): Comer FI, et al. 2001. Anal. Biochem. 293:169. Hart GW, et al. 1995. Adv. Exp. Med. Biol. 376:115. (ELISA, WB, IP) Anthonisen EH, et al. 2010. J. Biol. Chem. 285:1607. (WB) Dias WB, et al. 2009. J. Biol. Chem. 32:21327. (WB) Wang Z, et al. 2007. Mol. Cell Proteomics 8:1365. (IP) Kim SJ, et al. 2003. J. Biol. Chem. 43:41890. (IP) Kneass ZT, Marchase RB. 2004. J. Biol. Chem. 44:45759. (WB, ICC, IF)
Product Citations: Durst MA, et al. 2019. PLoS One. 14:e0210305. PubMed Kuo WL, et al. 2021. Diagnostics (Basel). 11:. PubMed Liu Y, et al. 2020. Nat Commun. 4.554166667. PubMed Basehore SE, et al. 2021. Circ Res. 129:1054. PubMed Jean-Louis T, et al. 2018. Neurobiol Aging. 62:130. PubMed
RRID: AB_2629520 (BioLegend Cat. No. 838004)
Structure: 1046 amino acids with a molecular weight of 117 kD.
Distribution: Cytosol, mitochondrion, nucleus, plasma membrane, and cytoskeleton.
Function: Catalyzes the transfer of a single N-acetylglucosamine from UDP-GlcNAc to a serine or threonine residue in cytoplasmic and nuclear proteins resulting in their modification with a beta-linked N-acetylglucosamine (O-GlcNAc), Glycosylates a large and diverse number of proteins including histone H2B, AKT1, EZH2, PFKL, KMT2E/MLL5, MAPT/TAU and HCFC1, Can regulate their cellular processes via cross-talk between glycosylation and phosphorylation or by affecting proteolytic processing.
Biology Area: Cell Biology, Transcription Factors
Gene ID: NA
Clone: CTD110.6
Regulatory Status: RUO
Other Names: O-Linked N-Acetylglucosamine
Isotype: Mouse IgM, κ