Biolegend, 838101, Anti-CD171 (L1CAM) Antibody (Previously Covance catalog# MMS-172R), 500microl

L1CAM, also known as CD171, is a neuronal cell adhesion molecule involved in axon guidance and cell migration with a strong implication in treatment-resistant cancers. This cell adhesion molecule plays an important role in nervous system development, including neuronal migration, and differentiation. Mutations in the gene cause three X-linked neurological syndromes known by the acronym CRASH (corpus callosum hypoplasia, retardation, aphasia, spastic paraplegia and hydrocephalus). CD171 has been shown to function as a cell adhesion molecule mediating homotypic and heterotypic cell-cell interactions in neuronal myelination, neurite outgrowth and regeneration.
500microl
Verified Reactivity: Human, Mouse, Rat
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: This monoclonal antibody was raised against purified chick L1, and binds to the L1 cytoplasmic domain at about T1172 (adjacent to the YRSL sorting signal).
Preparation: Ascites
Concentration: The concentration is not quantified as this product is sold as undiluted crude mouse ascites fluid. The concentration might vary from lot-to-lot and an estimated concentration would be 1-3 mg/ml.
Storage & Handling: Store at -20°C. It is suggested that the total volume be divided into useable aliquots upon initial thaw.
Application: WB - Quality testedICC, IP, IHC-P - Reported in the literature, not verified in house
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested dilution for this reagent is 1:2000. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: Phosphorylation of either T1172 or Y1176 strongly inhibits binding of 74-5H7 to the L1 cytoplasmic domain.
Application References(PubMed link indicates BioLegend citation): Kamiguchi H, et al. 1998. J. Neurosci. 18(14):5311-21. Lemmon V, et al. 1989. Neuron. 2(6):1597-603. Yip PM, Siu CH. 2001. J. Neurochem. 76(5):1552-64. Schaefer AW, et. al. 2002. J. Cell Biol. 157:1223. (WB, IHC-P, IP) Beer S, et. al. 1999. J. Cell Sci. 112:2667. (IP) Itoh K, et. al. 2005. Mol. Cell Neurosci. 29:245. (WB, IP) Zheng XJ, et. al. 2005. Environ. Technol. 26:1073. (ICC) Chen SY, et. al. 2001. FASEB. J. 15:1649. (IHC-P) PubMed Itoh K, et. al. 2004. J. Cell Biol. 165:145. (WB) Cohen NR, et. al. 1998. Curr. Biol. 8:26. (WB)
Product Citations: Chen S, et al. 2001. FASEB J. 15:1649-1561. PubMed Hu J, et al. 2022. Cell Death Dis. 13:48. PubMed Kleene R, et al. 2021. J Neurochem. 157:1102. PubMed Girbes Minguez M, et al. 2020. FASEB J. 34:9869. PubMed
RRID: AB_2565388 (BioLegend Cat. No. 838101)
Structure: Expected MW: 200-220 kD
Biology Area: Cell Biology, Neuroscience, Synaptic Biology
Molecular Family: Adhesion Molecules
Gene ID: 3897
UniProt: View information about CD171 on UniProt.org
Clone: 74-5H7
Regulatory Status: RUO
Other Names: Neural cell adhesion molecule L1, antigen identified by monoclonal antibody R1
Previously: Covance Catalog# MMS-172R
Isotype: Mouse IgG1, κ