Biolegend, 841001, Anti-Neurofilament M (NF-M) Antibody (Previously Covance catalog# PRB-575C), 100microl

Neurofilaments can be defined as the intermediate or 10nm diameter filaments found in neuronal cells. They are composed a mixture of subunits which often includes the neurofilament triplet proteins, NF-L, NF-M and NF-H. Neurofilaments may also include peripherin, a-internexin, nestin and in some cases vimentin. Antibodies in the various neurofilament subunits are very useful cell type markers since the proteins are quite abundant, biochemically stable.
100microl
Verified Reactivity: Human, Rat, Mouse
Antibody Type: Polyclonal
Host Species: Rabbit
Immunogen: The immunogen was the extreme carboxyterminal region of rat NF-M, which was expressed in E. coli and purified by ion exchange chromatograpy.
Preparation: Serum
Storage & Handling: Store at -20°C. Upon initial thawing, apportion into working aliquots and store at -20°C. Avoid repeated freeze-thaw cycles to prevent denaturing the antibody.
Application: IHC-P - Quality tested WB - Verified
Recommended Usage: Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a dilution range of 1:200 - 1:500 is suggested. For Western blotting, the suggested use of this reagent is 1:5000 - 1:10000. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: The production and characterization of this antiserum similar to monoclonal NF-M clone 3H11. This product may contain other non-IgG subtypes.
Application References(PubMed link indicates BioLegend citation): Harris J, Ayyub C, Shaw G. A molecular dissection of the carboxyterminal tails of the major neurofilament subunits NF-M and NF-H. J. Neurosci. Res. 30:47-62, 1991.
Product Citations: Vieira JR, et al. 2022. Neuron. 110:4074. PubMed Homma S, et al. 2023. Front Neurosci. 16:1009542. PubMed Min S, et al. 2020. Cell Reports. 29(8):2192-2201.e3.. PubMed Paredes I, et al. 2021. Nat Neurosci. 24:478. PubMed Xu R, et al. 2018. Nat Med. 24:823. PubMed Kirst C, et al. 2020. Cell. 180(4):780-795. PubMed
RRID: AB_2565457 (BioLegend Cat. No. 841001)
Structure: Expected MW: 145-160 kD
Cell Type: Mature Neurons
Biology Area: Cell Biology, Neuroscience, Neuroscience Cell Markers
Molecular Family: Intermediate Filaments
Gene ID: 4741
UniProt: View information about Neurofilament M on UniProt.org
Clone: Poly28410
Regulatory Status: RUO
Other Names: 160 kD Neurofilament Protein, Neurofilament Triplet M Protein, Neurofilament-3 (150 Kd Medium), Neurofilament, Medium Polypeptide 150kD
Previously: Covance Catalog# PRB-575C
Isotype: Rabbit Polyclonal IgG