Biolegend, 844301, Purified anti-CRISPR (CAS9) Antibody, 100μg

Cas9 is an RNA-guided DNA endonuclease enzyme associated with the CRISPR (Clustered Regularly Interspersed Palindromic Repeats) adaptive immunity system in Streptococcus pyogenes , among other bacteria. Cas9 interrogates and cleaves foreign DNA, such as invading bacteriophage DNA or plasmid DNA. Cas9 performs this interrogation by unwinding foreign DNA and checking if it is complementary to the 20 basepair spacer region of the guide RNA. If the DNA substrate is complementary to the guide RNA, Cas9 cleaves the invading DNA. In this sense, the CRISPR-Cas9 mechanism has a number of parallels with the RNA interference (RNAi) mechanism in eukaryotes. This product was carefully developed for performance and specificity using the following products from Horizon Discovery, a Revvity company: HAP1 Cas9 Stable Cell Line (Horizon Cat No. HD Cas9-011) HAP1 WT (Horizon Cat. No. C631)
100μg
Verified Reactivity: S. Pyogenes
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Recombinant S. pyogenes Cas9 Protein (N-terminal 250 amino acids) expressed in E. coli.
Formulation: Phosphate-buffered solution, pH 7.2.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 1.0 mg/mL
Storage & Handling: This antibody should be handled aseptically as it is free of preservatives such as Sodium Azide. Store this antibody undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check the vial label or CoA to find the proper storage conditions.
Application: ICC - Quality tested WB, IP, ICFC - Verified
Recommended Usage: Each lot of this antibody is quality control tested by immunocytochemistry. For immunocytochemistry, the suggested working dilution is 1.0 - 10 µg/mL. For western blotting, the suggested use of this reagent is 0.5 - 1.0 µg per mL. For flow cytometric staining using our Cyto-Fast™ Fix/Perm Buffer Set, the suggested use of this reagent is ≤ 0.062 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application. For use of this antibody in intracellular flow cytometry (ICFC), we recommend fixation and permeabilization using Cyto-Fast™ Fix-Perm Buffer set (Cat. No. 426803). Similar results were also obtained using True-Phos™ Perm Buffer (Cat. No. 425401).
Product Citations: Shang P, et al. 2023. Nat Commun. 14:2615. PubMed Lucas CD, et al. 2022. Sci Immunol. 7:eabm4032. PubMed Roy IM, et al. 2022. iScience. 25:105171. PubMed Jo DH, et al. 2023. Mol Ther Nucleic Acids. 31:16. PubMed Usman WM, et al. 2018. Nat Commun. 9:278. PubMed Zafra MP, et al. 2018. Nat Biotechnol. 36:888. PubMed Smeenk L, et al. 2021. Cancer Discov. 11:2868. PubMed Jang HK, et al. 2021. Sci Adv. 7:. PubMed Geisinger JM, et al. 2020. Nucleic Acids Res. 48:9067. PubMed Clements KE, et al. 2020. Nat Commun. 4.706944444. PubMed Habib O, et al. 2022. Nucleic Acids Res. 50:1187. PubMed Siadous FA, et al. 2020. Autophagy. :1. PubMed Scheidmann MC, et al. 2022. Cancer Res. 82:681. PubMed Freitas GP, et al. 2021. Gene Ther. 28:748. PubMed Schleicher EM, et al. 2020. PLoS Genet. 16:e1009176. PubMed Henriksson J, et al. 2019. Cell. 176:882. PubMed
RRID: AB_2749904 (BioLegend Cat. No. 844302) AB_2565570 (BioLegend Cat. No. 844301)
Structure: The architectures of Cas9 enzymes define nucleic acid binding clefts to form a central channel where DNA substrates are bound.
Function: The Cas9 protein from S. pyogenes is part of the CRISPR/Cas9 system of acquired immunity that bacteria have evolved to destroy foreign DNA elements, such as bacteriophages.
Biology Area: Cell Biology, Neuroscience
Antigen References: 1. Mali P, et al. 2013. Science 339:823.
Gene ID: 901176
UniProt: View information about CRISPR on UniProt.org
Clone: 7A9
Regulatory Status: RUO
Other Names: CRISPR associated protein 9, SpyCas9
Isotype: Mouse IgG1, κ