Biolegend, 844602, Purified anti-β Catenin 1 (CTNNB1) Antibody, 100μg

β-catenin is part of a complex of proteins that constitute adherens junctions (AJs). AJs are necessary for the creation and maintenance of epithelial cell layers by regulating cell growth and adhesion between cells. The encoded protein also anchors the actin cytoskeleton and may be responsible for transmitting the contact inhibition signal that causes cells to stop dividing once the epithelial sheet is complete. β-catenin also plays a key role in Wnt signaling pathways and thus is involved in neural differentiation, synaptic plasticity, neurodegenerative disease, and prevention of apoptosis. Finally, this protein binds to the product of the adenomatous polyposis coli (APC) gene, which is mutated in the adenomatous polyposis of the colon. Mutations in this gene are a cause of colorectal cancer (CRC), pilomatrixoma (PTR), medulloblastoma (MDB), and ovarian cancer.
100μg
Verified Reactivity: Human, Mouse, Rat
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Recombinant β-catenin (N-terminal fragment) fused to MBP.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested IHC-P, ICFC - Verified
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1.0 - 10 µg/mL. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration of 10 µg/mL is suggested. For intracellular flow cytometry using our True-Nuclear™ Transcription Factor Staining Protocol, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: Additional reported applications (for the relevant formats) include: immunfluorescence microscopy4 and immunohistochemical staining3. For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 844603) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01 EU/µg).
Application References(PubMed link indicates BioLegend citation): Sacco P, et al. 1995. J. Biol. Chem. 270:20201. (WB) Johnson KR, et al. 1993. Exp. Cell Res. 207:252. Gupta K, et al. 2012. J. Ped. Hem. Onc. 34:320. (IHC-P) Radice G, et al. 1997. Dev. Bio. 181:64. (IHC-P)
Product Citations: Du W, et al. 2024. PLoS Genet. e1011326:20. PubMed Davies SP, et al. 2019. Cell Rep. 29:1610. PubMed Huang F, et al. 2018. Oncol Rep. 39:809. PubMed Nanna AR, et al. 2020. Nucleic Acids Res. 48:5281. PubMed
RRID: AB_2750041 (BioLegend Cat. No. 844601) AB_2565803 (BioLegend Cat. No. 844602)
Structure: 88 kD armadillo repeat containing protein with an unstructured N and C terminal.
Distribution: β-catenin localizes to cell-cell contacts (adherens junctions) and the cell membrane as well as the cytoplasmic pool of protein. β-catenin can also translocate to the nucleus and is in interphase found in puncta at the proximal end of centrioles. In mitosis, it localizes to the centrosomes at the spindle.
Function: Wnt signaling pathway, regulation of cell-cell adhesion, neurogenesis, transcription, transcription regulation, early embryonic patterning, asymmetric cell division, stem cell renewal, and the epithelial to mesenchymal transition.
Interaction: Wnt signaling pathway, apoptotic cleavage of cellular proteins, and EphB-EphrinB signaling.
Biology Area: Cell Adhesion, Cell Biology, Neuroscience, Signal Transduction, Synaptic Biology
Molecular Family: Adhesion Molecules
Antigen References: 1. Hirano S, et al. 2012. Physiol. Rev. 92:597.
Gene ID: 1499
UniProt: View information about beta Catenin 1 on UniProt.org
Clone: 12F7
Regulatory Status: RUO
Other Names: Catenin (Cadherin-Associated Protein), Beta 1, 88 kD, CTNNB1, TNNB, MRD19, armadillo, catenin beta-1
Isotype: Mouse IgG1, κ