Biolegend, 845002, Purified anti-CD209/CD299 (DC-SIGN/L-SIGN) Antibody, 100μg

DC-SIGN is a C-type lectin that has a high affinity for the ICAM3 molecule. It binds various microorganisms by recognizing high-mannose-containing glycoproteins on their envelopes. It especially functions as a receptor for several viruses such as HIV and Hepatitis C. L-SIGN is a transmembrane receptor and is referred to as L-SIGN because of its expression in the endothelial cells of the lymph nodes and liver. The protein is involved in the innate immune system and recognizes numerous evolutionarily divergent pathogens ranging from parasites to viruses. The protein is organized into three distinct domains: an N-terminal transmembrane domain, a tandem-repeat neck domain, and a C-type lectin carbohydrate recognition domain.
100μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: DC-SIGN DNA.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: Clone 14E3G7 has been shown to recognize both CD209 and CD299 transfectants.1
Application References(PubMed link indicates BioLegend citation): Granelli-Piperno A, et al. 2005. J. Immunol. 175:4265.
Product Citations: Lempp FA, et al. 2021. Nature. 598:342. PubMed
RRID: AB_2566004 (BioLegend Cat. No. 845002)
Structure: DC-SIGN is a 44 kD type II transmembrane glycoprotein and a member of the C-type lectin family. DC-SIGNR (DC-SIGN-related molecule) is a type II integral membrane protein that is 77% identical to DC-SIGN.
Distribution: CD209 is expressed on myeloid dendritic cells, placental macrophages, and liver and placental endothelial cells. CD299 is expressed on liver sinusoidal endothelial cells, lung and placental endothelial cells, type 2 alveolar cells, and endothelial cells of lymph nodes.
Function: CD299 mediates interactions between dendritic cells and resting T cells and has a high affinity for HIV-1 gp120. CD209 plays a critical role in capturing and internalizing pathogens. LSP1 (leukocyte-specific protein 1) interacts with the cytoplasmic domain of CD209 and mediates the transport of HIV to the proteasome.
Interaction: CD209 is a receptor of viruses such as HIV-1, West Nile Virus, hepatitis C virus, some bacteria, and parasites. CD299 binds carbohydrate structures of pathogens such as hepatitis C virus, Mycobacterium, etc.
Ligand/Receptor: CD209 binds to ICAM-3 (CD50), ICAM-2 (CD102), and Butyrophilin (BTNA2A1). CD299 is a receptor for ICAM-3 (CD50).
Cell Type: Dendritic cells, Endothelial cells, Macrophages
Biology Area: Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience
Molecular Family: CD Molecules
Antigen References: 1. Fauci A. 1996. Nature 384:529. 2. Yokoyama-Kobayashi M, et al. 1999. Gene 228:161. 3. Soilleux EJ, et al. 2000. J. Immunol. 165:2937. 4. Pohlmann S, et al. 2001. Proc. Natl. Acad. Sci. USA 98:2670. 5. Bashirova AA, et al. 2001. J. Exp. Med. 193:671. 6. Lai WK, et al. 2006. Am. J. Pathol. 169:200.
Gene ID: 3083510332
UniProt: View information about CD209 on UniProt.org
Clone: 14E3G7
Regulatory Status: RUO
Other Names: For CD209: CDSIGN, CLEC4L, DC-SIGN, DC-SIGN1, For CD299: LSIGN, CD209L, L-SIGN, DCSIGNR, HP10347, DC-SIGN2, DC-SIGNR
Isotype: Mouse IgG2b, κ